Expression of the DNAM-1 ligands, Nectin-2 (CD112) and poliovirus receptor (CD155), on dendritic cells: relevance for natural killer-dendritic cell interaction
Expression of the DNAM-1 ligands, Nectin-2 (CD112) and poliovirus receptor (CD155), on dendritic cells: relevance for natural killer-dendritic cell interaction
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DOI：
10.1182/blood-2005-07-2696
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发表时间：
2006-03-01
期刊：
影响因子：
20.3
通讯作者：
Bottino, C
中科院分区：
文献类型：
--
作者：
Pende, D;Castriconi, R;Bottino, C
在这项研究中，我们证明了dnam -1触发受体及其配体，脊髓灰质炎病毒受体（PVR）和Nectin-2参与自然杀伤（NK）细胞介导的树突状细胞（DCs）裂解。与单核细胞相比，这两种配体的表面表达在dc中上调。在脂多糖（LPS）、聚I:C、鞭毛蛋白和CD40L等不同刺激诱导DC成熟后，其密度达到最大。免疫组织化学分析和共聚焦显微镜显示DCs在淋巴结中表达DNAM-1配体，它们位于滤泡旁t细胞区并包围高内皮小静脉。值得注意的是，在细胞溶解实验中，DNAM-1与NKp30共同参与nk介导的未成熟和成熟dc的杀伤，DNAM-1的贡献程度似乎与其特异性配体PVR和Nectin-2的表面密度相关。
In this study, we demonstrate the involvement of DNAM-1-triggering receptor and its ligands, poliovirus receptor (PVR) and Nectin-2, in natural killer (NK) cell-mediated lysis of dendritic cells (DCs). The surface expression of both ligands was up-regulated in DCs as compared to monocytes. It reached maximal densities after DC maturation induced by different stimuli including lipopolysaccharide (LPS), poly I:C, flagellin, and CD40L. Both immunohistochemical analysis and confocal microscopy revealed expression of DNAM-1 ligands by DCs in lymph nodes in which they were localized in the parafollicular T-cell region and surrounded the high endothelial venules. Remarkably, in cytolytic assays, DNAM-1 cooperated with NKp30 in the NK-mediated killing of both immature and mature DCs and the degree of contribution of DNAM-1 appeared to correlate with the surface densities of its specific ligands PVR and Nectin-2.