Streptomyces kanasensis sp nov., an Antiviral Glycoprotein Producing Actinomycete Isolated from Forest Soil Around Kanas Lake of China
Streptomyces kanasensis sp nov., an Antiviral Glycoprotein Producing Actinomycete Isolated from Forest Soil Around Kanas Lake of China
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DOI：
10.1007/s00284-015-0900-0
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发表时间：
2015-12-01
影响因子：
2.6
通讯作者：
Feng, Juntao
中科院分区：
文献类型：
--
作者：
Han, Lirong;Zhang, Guoqiang;Feng, Juntao
从我国喀纳斯湖周边森林土壤中分离到一株丝状放线菌，命名为ZX 01（T）。对菌株ZX 01（T）进行了多相分类学研究。分离物的化学和形态特征与链霉菌属的物种相似。对几乎完整的16 S rRNA基因序列的分析将菌株ZX 01（T）置于链霉菌属中，其中它与该属的公认种形成了独特的系统系。该菌株与熏衣草叶链霉菌NBRC 12882（T）、S. luridus NBRC 12793（T）（99.0%）、S.熏衣草色NBRC 12881（T）（99.0%）、S. gobitricini NBRC 15419（T）（99.0%）和S. Roseolilacinus NBRC 12815（T）（98.9%）。菌株ZX 01（T）与上述菌株的DNA-DNA相关性较低，分别为54.0、50.0、60.0、66.7和50.4%。许多表型特性也使分离物能够与链霉菌属的相关物种区分开来。因此，建议将菌株ZX 01（T）归为链霉菌属中的一个新种--卡那链霉菌Streptomyceskanasensissp. nov.的模式菌株，其模式菌株为ZX 01（T）（= CGMCC 4893（T）=JCM 30232（T））。
A filamentous actinomycete, designated strain ZX01(T), was isolated from forest soil around Kanas Lake of China. A polyphasic taxonomic study was carried out to establish the status of strain ZX01(T). Chemical and morphological properties of the isolate were similar to those of species of the genus Streptomyces. Analysis of the almost complete 16S rRNA gene sequence placed strain ZX01(T) in the genus Streptomyces where it formed a distinct phyletic line with recognized species of this genus. The strain exhibited the highest sequence similarities to Streptomyces lavendofoliae NBRC 12882(T) (99.1 %), S. luridus NBRC 12793(T) (99.0 %), S. lavendulocolor NBRC 12881(T) (99.0 %), S. gobitricini NBRC 15419(T) (99.0 %), and S. roseolilacinus NBRC 12815(T) (98.9 %). Low DNA-DNA relatedness values of 54.0, 50.0, 60.0, 66.7, and 50.4 %, respectively, were found between strain ZX01(T) and corresponding strains above. A number of phenotypic properties also enabled the isolate to be differentiated from related species of the genus Streptomyces. Therefore, it is proposed that strain ZX01(T) should be classified as the type strain of a novel species in the genus Streptomyces, Streptomyces kanasensis sp. nov. The type strain is ZX01(T) (= CGMCC 4893(T) =JCM 30232(T)).