Ultrasensitive quartz crystal microbalance sensors for detection of M13-Phages in liquids
Ultrasensitive quartz crystal microbalance sensors for detection of M13-Phages in liquids
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DOI：
10.1016/s0956-5663(01)00220-2
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发表时间：
2001-12-01
影响因子：
12.6
通讯作者：
Drost, S
中科院分区：
文献类型：
--
作者：
Uttenthaler, E;Schräml, M;Drost, S
石英晶体微天平（QCM）传感器被广泛用于测定液体性质或探测界面过程。对于某些应用，与其他生物传感器方法相比，通常使用的QCM传感器的灵敏度（5-20 MHz）是有限的。在这项研究中，超灵敏的QCM传感器的谐振频率从39到110 MHz的测量在液相中。QCM的基本传感器效应是在测量期间由于质量在涂覆表面上的结合而导致振荡石英晶体的谐振频率的降低。QCM传感器的灵敏度随着谐振频率的增加而强烈增加，因此，随着敏感区域的厚度的减小而增加。本研究中使用的新型超灵敏QCM传感器是基于化学研磨的剪切模式石英晶体，其仅在空白的中心被蚀刻。形成具有厚的、机械稳定的外环的薄石英膜。使用病毒特异性单克隆抗体和M13-噬菌体的免疫测定显示，与标准19 MHz石英晶体相比，56 MHz石英晶体的信噪比提高了6倍以上，检测限提高了200倍。甘油/水混合物的声学性质的探测导致灵敏度的增加，这是在非常好的协议与理论。化学研磨的QCM传感器大大提高了生物传感和探测声学特性的灵敏度，因此，为QCM传感器提供了有趣的新应用领域。(C)2001 Elsevier Science B. V.保留所有权利。
Quartz crystal microbalance (QCM) sensors are widely used for determining liquid properties or probing interfacial processes. For some applications the sensitivity of the QCM sensors typically used (5-20 MHz) is limited compared with other biosensor methods. In this study ultrasensitive QCM sensors with resonant frequencies from 39 to 110 MHz for measurements in the liquid phase are presented. The fundamental sensor effect of a QCM is the decrease of the resonant frequency of an oscillating quartz crystal due to the binding of mass on a coated surface during the measurement. The sensitivity of QCM sensors increases strongly with an increasing resonant frequency and, therefore, with a decreasing thickness of the sensitive area. The new kind of ultrasensitive QCM sensors used in this study is based on chemically milled shear mode quartz crystals which are etched only in the center of the blank. forming a thin quartz membrane with a thick, mechanically stable outer ring. An immunoassay using a virus specific monoclonal antibody and a M13-Phage showed an increase in the signal to noise ratio by a factor of more than 6 for 56 MHz quartz crystals compared with standard 19 MHz quartz crystals, the detection limit was improved by a factor of 200. Probing of acoustic properties of glycerol/water mixtures resulted in an increase in sensitivity, which is in very good agreement with theory. Chemically milled QCM sensors strongly improve the sensitivity in biosensing and probing of acoustic properties and, therefore, offer interesting new application fields for QCM sensors. (C) 2001 Elsevier Science B.V. All rights reserved.