Antidiabetic and hypolipidemic effects of adropinin streoptozotocin-induced type 2 diabetic rats
Antidiabetic and hypolipidemic effects of adropinin streoptozotocin-induced type 2 diabetic rats
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DOI：
10.4149/bll_2016_020
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发表时间：
2016-01-01
影响因子：
1.5
通讯作者：
Kokdasgil, H.
中科院分区：
文献类型：
--
作者：
Akcilar, R.;Kocak, F. E.;Kokdasgil, H.
背景技术背景：方法：采用高脂饲料和链脲佐菌素（STZ; 35 mg/kg/b.w.）建立2型糖尿病（T2 DM）大鼠模型，观察阿霉素（Adropin）对T2 DM大鼠糖脂代谢的影响。STZ诱导后7天，糖尿病大鼠随机用阿霉素（2.1 μ g/kg/天，腹膜内）治疗10天。该研究涉及评价生化参数，包括血糖、总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、碱性磷酸酶（ALP）和γ谷氨酰转移酶（GGT）活性。采用逆转录-聚合酶链反应（RT-PCR）检测胰腺组织中肿瘤坏死因子-α（TNF-α）、白细胞介素6（IL-6）和诱导型一氧化氮合酶（iNOS）mRNA的表达。Adropin治疗显示血糖水平、HbA 1c（%）、HOMA-β显著降低，HOMA-6、血清胰岛素水平升高。腹腔注射阿霉素可降低血清TC、TG、LDL-C水平，升高HDL-C水平。Adropin还能有效地改善TNF-α、IL-6和iNOSmRNA表达的改变。结论：Adropin具有抗糖尿病和降血脂的作用。2，图3，参考文献32）。
BACKGROUND: To examine the effects of adropin on glucose and lipid metabolism in a rat model of Type 2 diabetes mellitus (T2DM).METHODS: T2DM were established using high-fat diet and streptozocin (STZ; 35 mg/kg/b.w.). Seven days after STZ induction, diabetic rats were randomly treated with adropin (2.1 mu g/kg/day intraperitonealy) for 10 days. The study involved the evaluation of biochemical parameters, including blood glucose, total cholesterol (TC), triglycerides (TG), low-density lipoprotein-cholesterol (LDL-C), high-density lipoprotein-cholesterol (HDL-C), aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and gamma glutamyl transferase (GGT) activities. Additionally, Tumor necrosis factor-alpha (TNF-alpha), interleukin 6 (IL-6) and inducible nitric oxide synthase (iNOS) mRNA gene expressions in pancreas tissue were determined by reverse transcription-polymerase chain reaction.RESULTS: The serum levels of insulin and adropin were determined by ELISA. Treatment with adropin showed a significant reduction in blood glucose levels, HbA1c (%), HOMA-beta and increase in HOMA-6, serum insulin levels. In addition, intraperitoneal adropin application can reduce serum levels of TC, TG, LDL-C, and increase level of HDL-C. Adropin also effectively ameliorated the alterations in TNF-alpha, IL-6 and iNOS mRNA expression.CONCLUSION: The present study indicates that the adropin possesses antidiabetic and antidyslipidemic effects in T2DM (Tab. 2, Fig. 3, Ref. 32).