Use of a yeast-based membrane protein expression technology to overexpress drug resistance efflux pumps.
Use of a yeast-based membrane protein expression technology to overexpress drug resistance efflux pumps.
复制标题
使用基于酵母的膜蛋白表达技术来过度表达耐药性外排泵。
DOI：
10.1007/978-1-60761-820-1_15
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Cannon,RichardD
中科院分区：
文献类型：
--
作者：
Lamping,Erwin;Cannon,RichardD
唑类抗真菌药物广泛用于治疗口腔真菌感染。不幸的是，真菌可能对这些药物产生耐药性。这种耐药可能是由于细胞色素P450 14α-羊毛甾醇脱甲基酶（也称为ERG 11或CYP 51）的过表达或突变，和/或膜定位多药外排泵的过表达。我们已经开发了一个异源膜蛋白表达系统，可用于研究这些蛋白质的结构和功能的非致病性，遗传稳定，和多功能的真核模式生物，酿酒酵母。在本章中，我们描述了用于在酿酒酵母中表达白色念珠菌外排泵Cdr 1 p的技术。
Azole antifungal drugs are used widely to treat people with oral fungal infections. Unfortunately, fungi can develop resistance to these drugs. This resistance can be due to the overexpression or mutation of cytochrome P450 14α-lanosterol demethylase, also known asERG11orCYP51, and/or the overexpression of membrane-located multidrug efflux pumps. We have developed a heterologous membrane protein expression system that can be used to study the structure and function of these proteins in the non-pathogenic, genetically stable, and versatile eukaryotic model organism,Saccharomyces cerevisiae. In this chapter we describe the techniques used to express theCandida albicansefflux pump Cdr1p inS. cerevisiae.