The micro-structure and biomechanics of eyelid tarsus
The micro-structure and biomechanics of eyelid tarsus
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DOI：
10.1016/j.jbiomech.2021.110911
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发表时间：
2022-01-22
影响因子：
2.4
通讯作者：
Ye，Juan
中科院分区：
文献类型：
--
作者：
Gao，Qi;Xu，Peifang;Ye，Juan
眼睑睑板是睑板腺周围的纤维软骨细胞外基质，为眼睑提供结构支撑，并在眼表的完整性中发挥重要作用。既往尚未有研究探讨眼睑睑板的微观结构与功能之间的关系。为了研究跗骨细胞外基质的结构和生物力学特性，对兔跗骨进行苏木精和伊红（H&E）、MASSON和Verhoeff's Van Gieson（EVG）染色，并用扫描电镜分析跗骨细胞外基质中胶原蛋白和弹性蛋白纤维的分布。收集跗骨条并用英斯特朗万能试验机进行单轴拉伸试验。该研究纳入了 15 个跗骨样本的数据。初始拉伸模量为2.554±1.453Mpa，最终拉伸模量为23.554±3.657Mpa，延伸率为35.47±7.46%。胶原纤维在睑板腺周围形成片层的外围层，而弹性蛋白纤维在水平和矢状切面中以平行排列方式组织，并在睑板腺周围以交叉排列方式组织。拉伸试验后，弹性蛋白纤维被拉伸并垂直于胶原纤维片层的方向排列。这项研究的结果表明，由胶原蛋白-弹性蛋白网络形成的细胞外基质结构有助于眼睑睑板的非线性机械特性。
Eyelid tarsus is a fibrocartilagenous extracellular matrix around meibomian glands providing structural support to eyelids and play important roles in the integrity of the ocular surface. There are no previous studies investigating the relationship between micro-structure and function of eyelid tarsus. To investigate the structure of extracellular matrix and the biomechanical properties of tarsus, rabbit tarsus were stained with hematoxylin and eosin (H&E), MASSON and Verhoeff's Van Gieson (EVG), distribution of collagen and elastin fibers in tarsus extracellular matrix were analyzed with scanning electron microscopy. Tarsus strips were collected and went through uniaxial tensile test with an Instron Universal Testing Machine. Data from 15 tarsus samples were included in the study. The initial tensile modulus was 2.554 ± 1.453 Mpa, and the final tensile modulus was 23.554 ± 3.657 Mpa, with an extensibility of 35.47 ± 7.46%. Collagen fibers formed peripheral layers of lamellae around meibomian glands, while the elastin fibres were organized in a parallel arrangement in horizontal and sagittal section, and in a crossed arrangement around meibomian glands. After tensile test, elastin fibres were stretched and arranged perpendicular to the direction of the collagen fibril lamellae. The findings of this study suggest that the extracellular matrix structure formed by collagen-elastin network contributes to a nonlinear mechanical characteristic of eyelid tarsus.