Topological regulation of cell division in Escherichia coli involves rapid pole to pole oscillation of the division inhibitor MinC under the control of MinD and MinE
Topological regulation of cell division in Escherichia coli involves rapid pole to pole oscillation of the division inhibitor MinC under the control of MinD and MinE
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DOI：
10.1046/j.1365-2958.1999.01575.x
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发表时间：
1999-10-01
影响因子：
3.6
通讯作者：
Lutkenhaus, J
中科院分区：
文献类型：
--
作者：
Hu, ZL;Lutkenhaus, J
在大肠杆菌中，Z环在中间细胞的位置是通过min系统的作用来保证的，min系统阻止了存在于细胞两极的潜在分裂位点的使用。min的这种活性是通过分裂抑制剂MinC的作用来实现的，MinC由MinD激活并由MinE进行拓扑调节。在本研究中，我们使用功能性GFP-MinC融合蛋白来监测MinC的定位，我们发现GFP-MinC在其他Min蛋白不存在的情况下是胞质蛋白。添加与MinC相互作用的外周膜蛋白MinD，导致GFP-MinC出现在膜上。在MinD和MinE都存在的情况下，GFP-MinC在细胞的两半之间快速振荡。因此，MinC由其他Min产物定位，但以动态方式定位，使得其处于阻止Z环组装远离中间单元的位置。
Placement of the Z ring at midcell in Escherichia coli is assured by the action of the min system, which blocks usage of potential division sites that exist at the cell poles. This activity of min is achieved through the action of an inhibitor of division, MinC, that is activated by MinD and topologically regulated by MinE. In this study, we have used a functional GFP-MinC fusion to monitor the location of MinC, We find that GFP-MinC is a cytoplasmic protein in the absence of the other Min proteins. The addition of MinD, a peripheral membrane protein that interacts with MinC, results in GFP-MinC appearing on the membrane. In the presence of both MinD and MinE, GFP-MinC oscillates rapidly between the halves of the cell. Thus, MinC is positioned by the other Min products, but in a dynamic manner so that it is in position to inhibit Z ring assembly away from midcell.