Exclusion of spheres by agarose gels during agarose gel electrophoresis: dependence on the sphere's radius and the gel's concentration.
Exclusion of spheres by agarose gels during agarose gel electrophoresis: dependence on the sphere's radius and the gel's concentration.
复制标题
琼脂糖凝胶电泳过程中琼脂糖凝胶对球体的排除：取决于球体的半径和凝胶的浓度。
DOI：
10.1016/0003-2697(86)90591-9
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发表时间：
1986
影响因子：
2.9
通讯作者：
Hayes,SJ
中科院分区：
文献类型：
--
作者：
Serwer,P;Hayes,SJ
球体的琼脂糖凝胶电泳（半径= R）已用于确定琼脂糖凝胶的孔的有效半径（PE）（凝胶中琼脂糖的百分比= A）。在给定A下的PE值被认为是进入凝胶的最大球体的R。当把log PE作为log A的函数作图时，结果可以表示为：PE= 118 A − 0.74（0.2 ≤ A ≤ 4.0（PE单位为nm））。然而，数据表明该图中存在显著的非线性，当A从0.2增加到4.0时，PE与A关系的指数的大小增加约20%。根据这些数据，可以用未修饰的、未掺杂的琼脂糖形成的琼脂糖凝胶获得大至1500 nm和小至36 nm的PE，并且可用于电泳。
Agarose gel electrophoresis of spheres (radius = R) has been used to determine the effective radius (PE) of the pores of an agarose gel (percentage of agarose in a gel = A). The value of PEat a given A was taken to be the R of the largest sphere that enters the gel. When log PEis plotted as a function of log A, the results can be represented by: PE= 118A−0.74for 0.2 ≤ A ≤ 4.0 (PEin nm). However, the data suggest significant nonlinearity in this plot, the magnitude of the exponent of the PEvs A relationship increasing by about 20% as A increases from 0.2 to 4.0. From these data, PE's as big as 1500 nm and as small as 36 nm can be achieved with agarose gels formed with unmodified, unadulterated agarose and usable for electrophoresis.