Remodeling of the adventitia during coronary arteriogenesis
Remodeling of the adventitia during coronary arteriogenesis
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DOI：
10.1152/ajpheart.00478.2002
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发表时间：
2003-01-01
影响因子：
4.8
通讯作者：
Schaper, J
中科院分区：
文献类型：
--
作者：
Cai, WJ;Koltai, S;Schaper, J
我们研究了动脉外膜在适应性动脉形成中的作用，在冠状动脉侧支血管（CV）的主动生长阶段诱导的慢性闭塞的左回旋冠状动脉在犬心脏。我们使用电子显微镜和免疫共聚焦（IF）标记bFGF，基质金属蛋白酶（MMP）-2，MMP-9，组织型纤溶酶原激活物（tPA），其抑制剂（派-1），纤维连接蛋白（FN），Ki-67。平滑肌细胞和外膜成纤维细胞增殖明显。定量IF显示，CV中的外膜MMP-2、MMP-9和FN是正常血管（NV）的9.2、7.5和8.6倍，bFGF是5.1倍，派-1是3.4倍。成纤维细胞的数量增加了5倍CV，但弹性纤维含量是25倍，在NV比CV。CV周围毛细血管中血管周围肌细胞损伤和内皮型一氧化氮合酶的诱导表明CV扩张。得出的结论是，外膜激活与CV的发展相关，通过细胞增殖，生长因子的产生，诱导细胞外蛋白水解，从而有助于适应性动脉形成过程中的重塑。
We studied the role of the adventitia in adaptive arteriogenesis during the phase of active growth of coronary collateral vessels (CV) induced by chronic occlusion of the left circumflex coronary artery in canine hearts. We used electron microscopy and immunoconfocal (IF) labeling for bFGF, matrix metalloproteinase (MMP)-2, MMP-9, tissue-type plasminogen activator (tPA), its inhibitor (PAI-1), fibronectin (FN), and Ki-67. Proliferation of smooth muscle cells and adventitial fibroblasts was evident. Quantitative IF showed that adventitial MMP-2, MMP-9, and FN were 9.2-, 7.5-, and 8.6-fold, bFGF was 5.1-fold, and PAI-1 was 3.4- fold higher in CV than in normal vessels (NV). The number of fibroblasts was 5-fold elevated in CV, but the elastic fiber content was 25-fold greater in NV than in CV. Perivascular myocyte damage and induction of endothelial nitric oxide synthase in peri-CV capillaries indicate expansion of CV. It was concluded that adventitial activation is associated with the development of CV through cell proliferation, production of growth factors, and induction of extracellular proteolysis thereby contributing to remodeling during adaptive arteriogenesis.