Fructus Lycii and Salvia miltiorrhiza Bunge extract alleviate retinitis pigmentosa through Nrf2/HO-1 signaling pathway
Fructus Lycii and Salvia miltiorrhiza Bunge extract alleviate retinitis pigmentosa through Nrf2/HO-1 signaling pathway
复制标题
枸杞子和丹参提取物通过Nrf2/HO-1信号通路缓解视网膜色素变性
DOI：
10.1016/j.jep.2021.113993
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发表时间：
2021
影响因子：
5.4
通讯作者：
Peng Qinghua
中科院分区：
文献类型：
--
作者：
Ou Chen;Jiang Pengfei;Tian Ye;Yao Zhen;Yang Yijing;Peng Jun;Zeng Meiyan;Song Houpan;Peng Qinghua
本研究旨在探讨丹参提取物对视网膜色素变性（RP）的保护作用，并探讨丹参提取物是否通过调节Nrf 2/HO-1信号通路发挥其抗氧化作用。Rd 10小鼠作为RP模型，随后通过灌胃给予FS提取物治疗4周。处死动物后，进行组织病理学检查和暗视视网膜电图（ERG）分析。测定氧化应激标志物，并通过qRT-PCR评估Nrf 2和HO-1 mRNA的表达水平。结果大鼠海马神经元外核层（ONL）厚度、数量、a波振幅、b波振幅均明显增加，神经元的形态学改变与正常对照组相比有显著性差异（P <0.05）。FS提取物处理显著提高了超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的含量，降低了丙二醛（MDA）的含量。结论FS提取物可改善视网膜形态和功能，其机制可能与调节Nrf 2/HO-1通路抑制氧化反应有关。
Ethnopharmacological relevanceFructus Lycii and Salvia miltiorrhiza Bunge (FS) are popular Chinese herbs for the treatment of retinitis pigmentosa (RP).Aim of the studyThis study was to evaluate protective effects of FS extract on RP and to explore whether FS extract exerts its protective effects via oxidative stress by regulating Nrf2/HO-1 signaling pathway.Material and methodsFS extract were identified by UPLC chromatographic analysis. Rd10 mice as the model of RP, followed by a 4-week FS extract treatment by intragastric administration. After the animal sacrifice, histopathological examination and Scotopic electroretinography (ERG) analysis were assessed. The oxidative stress markers were determined and the expression levels of Nrf2 and HO-1 mRNA were evaluated by qRT-PCR. The expression and distribution of Nrf2 and HO-1 protein were determined by Western blot and immunohistochemistry.ResultsThe morphological changes of Outer nuclear layer(ONL) thickness and number of the ONL were observed with a significant increased, and the functional changes of a-amplitude and b-wave amplitude were measured with a markedly increased. Treatment with FS extract remarkably increased levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and decreased level of malondialdehyde (MDA). Moreover, FS extract up-regulated mRNA and protein expression of Nrf2 and HO-1.ConclusionsThis study indicated that FS extract can improve retinal morphology and function, which may have occurred through the regulation of the Nrf2/HO-1 pathway to inhibit the oxidative reaction.