Scrapie strains maintain biological phenotypes on propagation in a cell line in culture
Scrapie strains maintain biological phenotypes on propagation in a cell line in culture
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DOI：
10.1093/emboj/20.13.3351
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发表时间：
2001-07-02
期刊：
影响因子：
11.4
通讯作者：
Bostock, CJ
中科院分区：
文献类型：
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作者：
Birkett, CR;Hennion, RM;Bostock, CJ
牛海绵状脑病（BSE）及其人类等价物，变异型克雅氏病（vCJD），是由相同的传染性病原体菌株引起的，其与绵羊瘙痒病同源物的> 20个菌株相似，但不同，从单一培养的细胞中可以更好地了解分子菌株决定因素，而不是从整个动物研究中，其中不同的细胞靶向通常是一种菌株-相关特征。虽然一些细胞类型可以被不同的菌株感染，但尚未研究出现的菌株的表型。我们已经治愈了羊瘙痒病感染的克隆SMB细胞系与戊聚糖硫酸盐，稳定地重新感染它与不同菌株的羊瘙痒病，并表明，生物学特性和朊病毒蛋白特征的每一个原始菌株在这个单一的非神经元细胞类型忠实地繁殖。这些发现证实了羊瘙痒病菌株决定簇在分离的细胞中是稳定的和不依赖宿主的。
Bovine spongiform encephalopathy (BSE) and its human equivalent, variant Creutzfeldt-Jakob disease (vCJD), are caused by the same strain of infectious agent, which is similar to, but distinct from, > 20 strains of their sheep scrapie homologue, A better understanding of the molecular strain determinants could be obtained from cells in monoculture than from whole animal studies where different cell targeting is commonly a strain-related feature. Although a few cell types can be infected with different strains, the phenotypes of the emergent strains have not been studied. We have cured the scrapie-infected, clonal SMB cell line with pentosan sulfate, stably re-infected it with a different strain of scrapie and shown that biological properties and prion protein profiles characteristic of each original strain are propagated faithfully in this single non-neuronal cell type. These findings attest to the fact that scrapie strain determinants are stable and host-independent in isolated cells.