SAP97 promotes the stability of Nax channels at the plasma membrane
SAP97 promotes the stability of Nax channels at the plasma membrane
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DOI：
10.1016/j.febslet.2012.09.018
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发表时间：
2012-11-02
期刊：
影响因子：
3.5
通讯作者：
Noda, Masaharu
中科院分区：
文献类型：
--
作者：
Matsumoto, Masahito;Fujikawa, Akihiro;Noda, Masaharu
Na-x是一种钠水平传感器，用于检测大脑脑室周围器官中表达的体液。Na-x在羧基（C）-末端具有推定的PSD-95/Disc-large/ZO-1（PDZ）-结合基序。在这里，我们发现几个PDZ蛋白结合Na-x的PDZ阵列覆盖测定。其中突触相关蛋白97（SAP 97/DLG 1）在穹窿下器与Na-x共表达。在C6胶质母细胞瘤细胞中，Na-x的PDZ结合基序的破坏或SAP 97的耗尽导致细胞表面Na-x的减少，其被内吞作用的抑制剂减弱。这些结果表明SAP 97有助于质膜上Na-x通道的稳定。Na-x通过抗标签免疫共沉淀与SAP 97物理相互作用（查看互动）CNRasGEF通过蛋白质阵列与Na-x结合（查看相互作用）Na-x和SAP 97通过荧光显微镜共定位（查看相互作用）GIPC 1通过蛋白质阵列与Na-x结合（查看相互作用）ZO-1通过蛋白质阵列与Na-x结合（查看相互作用）SAP 97通过蛋白质阵列与Na-x结合（查看相互作用）Densin-180通过蛋白质阵列与Na-x结合（查看相互作用）β-1-syntrophin通过蛋白质阵列与Na-x结合（查看相互作用）ERBIN通过蛋白质阵列与Na-x结合（查看交互）Na-x通过下拉与SAP 97物理交互（View interaction）Lnx 1 binds to Na-x by protein array（View interaction）nNOS binds to Na-x by protein array（View interaction）（C）2012欧洲生物化学学会联合会。由Elsevier B出版。版权所有© 2016
Na-x is a sodium-level sensor for body fluids expressed in the circumventricular organs in the brain. Na-x has a putative PSD-95/Disc-large/ZO-1 (PDZ)-binding motif at the carboxyl (C)-terminus. Here we found that several PDZ proteins bind to Na-x by PDZ-array overlay assay. Among them, synapse-associated protein 97 (SAP97/DLG1) was coexpressed with Na-x in the subfornical organ. In C6 glioblastoma cells, destruction of the PDZ-binding motif of Na-x or depletion of SAP97 resulted in a decrease in cell-surface Na-x, which was attenuated with inhibitors of endocytosis. These results indicate that SAP97 contributes to the stabilization of Na-x channels at the plasma membrane.Structured summary of protein interactions:Na-x physically interacts with SAP97 by anti tag coimmunoprecipitation (View interaction)CNRasGEF binds to Na-x by protein array (View interaction)Na-x and SAP97 colocalize by fluorescence microscopy (View interaction)GIPC1 binds to Na-x by protein array (View interaction)ZO-1 binds to Na-x by protein array (View interaction)SAP97 binds to Na-x by protein array (View interaction)Densin-180 binds to Na-x by protein array (View interaction)Beta-1-syntrophin binds to Na-x by protein array (View interaction)ERBIN binds to Na-x by protein array (View interaction)Na-x physically interacts with SAP97 by pull down (View interaction)Lnx1 binds to Na-x by protein array (View interaction)nNOS binds to Na-x by protein array (View interaction) (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.