Transcriptome Analysis of a Female-sterile Mutant (fsm) in Chinese Cabbage (Brassica campestris ssp pekinensis)
Transcriptome Analysis of a Female-sterile Mutant (fsm) in Chinese Cabbage (Brassica campestris ssp pekinensis)
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大白菜（Brassica Campestris ssp pekinensis）雌性不育突变体（fsm）的转录组分析
DOI：
10.3389/fpls.2017.00546
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发表时间：
2017-04-10
影响因子：
5.6
通讯作者：
Feng, Hui
中科院分区：
文献类型：
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作者：
Huang, Shengnan;Liu, Zhiyong;Feng, Hui
雌性不育突变体是研究植物雌蕊发育的理想材料。在此，我们鉴定了一个大白菜雌性不育突变体fsm。该突变体是以大白菜DH系FT为材料，采用游离小孢子培养和甲基磺酸乙酯诱变相结合的方法获得的，遗传稳定。与野生型‘FT’相比，fsm植株雌蕊败育，花器官也相对较小。遗传分析表明，其表型受一对隐性核基因控制。形态学观察表明，雌性不育系中异常胚珠的存在可能影响正常受精过程，最终导致雌性不育。使用RNA-Seq对'FT'和fsm的花蕾进行比较转录组分析，共发现1，872个差异表达基因（DEG）。其中，鉴定了参与雌蕊发育的许多基因，如PRETTY FEW SEEDS 2（PFS 2）、温度诱导的脂质运载蛋白（TIL）、AGAMOUS-LIKE（AGL）和HECATE（HEC）。此外，DEG的GO和KEGG途径富集分析表明，在雌蕊发育期间，多种生物过程和代谢途径显着富集。此外，利用qRT-PCR技术分析了16个DEG基因的表达模式，其中包括4个雌蕊发育相关基因和12个花器官发育相关基因。在fsm中共特异性检测到31,272个单核苷酸多态性。这些结果有助于揭示大白菜雌蕊发育的调控机制。
Female-sterile mutants are ideal materials for studying pistil development in plants. Here, we identified a female-sterile mutant fsm in Chinese cabbage. This mutant, which exhibited stable inheritance, was derived from Chinese cabbage DH line 'FT' using a combination of isolated microspore culture and ethyl methanesulfonate mutagenesis. Compared with the wild-type line 'FT,' the fsm plants exhibited pistil abortion, and floral organs were also relatively smaller. Genetic analysis indicated that the phenotype of fsm is controlled by a single recessive nuclear gene. Morphological observations revealed that the presence of abnormal ovules in fsm likely influenced normal fertilization process, ultimately leading to female sterility. Comparative transcriptome analysis on the flower buds of 'FT' and fsm using RNA-Seq revealed a total of 1,872 differentially expressed genes (DEGs). Of these, a number of genes involved in pistil development were identified, such as PRETTY FEW SEEDS 2 (PFS2), temperature-induced lipocalin (TIL), AGAMOUS-LIKE (AGL), and HECATE (HEC). Furthermore, GO and KEGG pathway enrichment analyses of the DEGs suggested that a variety of biological processes and metabolic pathways are significantly enriched during pistil development. In addition, the expression patterns of 16 DEGs, including four pistil development-related genes and 12 floral organ development-related genes, were analyzed using qRT-PCR. A total of 31,272 single nucleotide polymorphisms were specifically detected in fsm. These results contribute to shed light on the regulatory mechanisms underlying pistil development in Chinese cabbage.