Fine structure of lipid-depleted mitochondria.
Fine structure of lipid-depleted mitochondria.
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DOI：
10.1083/jcb.32.1.193
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发表时间：
1967-01
期刊：
影响因子：
--
通讯作者：
Stoeckenius W
中科院分区：
文献类型：
--
作者：
Fleischer S;Fleischer B;Stoeckenius W
本文研究了丙酮水溶液抽提去脂的线粒体和亚线粒体囊泡的精细结构。线粒体膜的薄片耗尽超过95%的脂质保留单位膜结构。电子显微照片的光密度描记表明，提取的线粒体的单位膜，平均而言，比未提取的控制，并表现出更大的宽度变化。线粒体外膜丢失，80-95%的脂质从中提取。内膜颗粒存在于亚线粒体囊泡耗尽高达85%的脂质。然而，当超过95%的脂质被去除时，很少（如果有的话）颗粒仍然附着在膜上，但在背景中发现许多颗粒未附着。当脂质被恢复到脂质缺乏的制剂，线粒体膜被发现是缺乏内膜颗粒，但完全活跃的琥珀酸-细胞色素c还原酶活性。
The fine structure of mitochondria and submitochondrial vesicles depleted of their lipid by extraction with aqueous acetone was studied. Thin sections of mitochondrial membranes depleted of more than 95% of their lipid retained the unit membrane structure. Densitometer tracings of the electron micrographs showed that the unit membrane of extracted mitochondria was, on the average, wider than that of unextracted controls and showed a greater variation in width. The outer membrane was lost in mitochondria from which 80–95% of the lipids was extracted. Inner membrane particles were present on submitochondrial vesicles depleted of up to 85% of their lipids. However, when more than 95% of the lipid was removed, few, if any, particles remained attached to the membranes but many particles were found unattached in the background. When lipid was restored to lipid-deficient preparations, the mitochondrial membranes were found to be devoid of inner membrane particles but were fully active with respect to succinate-cytochrome c reductase activity.