Application of response surface methodology to cell immobilization for the production of palatinose
Application of response surface methodology to cell immobilization for the production of palatinose
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DOI：
10.1016/j.biortech.2006.09.046
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发表时间：
2007-11-01
影响因子：
11.4
通讯作者：
Lele, S. S.
中科院分区：
文献类型：
--
作者：
Mundra, Piyushkumar;Desai, Kiran;Lele, S. S.
以产帕拉金糖的大黄欧文氏菌NCPPB 1578为研究对象，采用基于多变量非线性模型的响应面法（RSM），研究了海藻酸钠浓度、细胞负载量和微球直径3个固定化参数的相互作用和优化。方差分析和统计参数计算表明，响应面可以有效地用于模拟和改善复杂系统，如细胞固定化。帕拉金糖产量提高了40%。当藻酸盐浓度为5%w/v，细胞负载量为gl（-1），珠粒直径为2.25 μ m时，在1h的一批中获得了140 mg/ml的最大产量。因此，E.通过应用响应面技术，成功地将漏芦NCPPB 1578固定在藻酸盐珠中，并随后提高帕拉金糖产率。(C)2006爱思唯尔有限公司版权所有。
Response surface methodology (RSM), based on multivariate non-linear model, was applied to study the interactions and optimization of the immobilization parameters for cell entrapment, namely alginate concentration, cell loading and bead diameter using Erwinia rhapontici NCPPB 1578 that produced palatinose. ANOVA analysis and statistical parameters calculations showed that RSM could be used effectively to model and improve a complex system like cell immobilization. Palatinose yield was increased by 40%. The maximum yield of 140 mg/ml was achieved in a batch of I h at alginate concentration of 5% w/v, cell loading of g l(-1) and 2.25 turn bead diameter. Thus, the E. rhapontici NCPPB 1578 immobilization in alginate bead and subsequent palatinose yield was successfully improved by application of RSM technique. (C) 2006 Elsevier Ltd. All rights reserved.