Genetic relatedness of commensal Escherichia coli from nursery pigs in intensive pig production in Denmark and molecular characterization of genetically different strains
Genetic relatedness of commensal Escherichia coli from nursery pigs in intensive pig production in Denmark and molecular characterization of genetically different strains
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DOI：
10.1111/jam.12840
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发表时间：
2015-08-01
影响因子：
4
通讯作者：
Olsen, J. E.
中科院分区：
文献类型：
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作者：
Herrero-Fresno, A.;Larsen, I.;Olsen, J. E.
目的确定丹麦集约化生产中仔猪大肠杆菌的遗传相关性以及毒力和抗生素抗性基因的存在。方法和结果随机挑选1000株大肠杆菌菌株的遗传多样性（N=50株分离株）来自培养的粪便样本通过重复基因外回文PCR（REP-PCR）分析了来自5个集约化丹麦猪场的10头猪（N=4头猪），检测到42个独特的REP-谱（相似性
AimsTo determine the genetic relatedness and the presence of virulence and antibiotic resistance genes in commensal Escherichia coli from nursery pigs in Danish intensive production.Methods and ResultsThe genetic diversity of 1000 E.coli strains randomly picked (N=50 isolates) from cultured faecal samples (N=4 pigs) from five intensive Danish pigs farms was analysed by repetitive extragenic palindromic-PCR (REP-PCR) and 42 unique REP-profiles were detected (similarity