High throughput sequencing identifies chilling responsive genes in sweetpotato (Ipomoea batatas Lam.) during storage
High throughput sequencing identifies chilling responsive genes in sweetpotato (Ipomoea batatas Lam.) during storage
复制标题
高通量测序鉴定了甘薯 (Ipomoea batatas Lam.) 储存期间的低温响应基因
DOI：
10.1016/j.ygeno.2018.05.014
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发表时间：
2019
期刊：
影响因子：
4.4
通讯作者：
Li Zongyun
中科院分区：
文献类型：
--
作者：
Xie Zeyi;Zhou Zhilin;Li Hongmin;Yu Jingjing;Jiang Jiaojiao;Tang Zhonghou;Ma Daifu;Zhang Baohong;Han Yonghua;Li Zongyun
甘薯（Ipomoea batatasL.）是全球重要的经济粮食作物。甘薯属于旋花科，原产于热带，但甘薯在贮藏过程中对冷胁迫非常敏感。本研究利用转录组测序技术研究甘薯贮藏期间对低温胁迫的反应。通过高通量测序总共产生了110，110个unigenes。差异表达基因（DEGs）分析表明，在低温条件下，有18,681个基因表达上调，21,983个基因表达下调。许多DEG与细胞膜系统、抗氧化酶、碳水化合物代谢和激素代谢有关，可能与甘薯的低温抗性有关。DEGs的存在为甘薯在低温贮藏条件下的生理生化后果提供了分子基础。本研究为通过遗传操作提高甘薯采后耐冷性提供了新的靶点。
Sweetpotato (Ipomoea batatasL.) is a globally important economic food crop. It belongs to Convolvulaceae family and origins in the tropics; however, sweetpotato is sensitive to cold stress during storage. In this study, we performed transcriptome sequencing to investigate the sweetpotato response to chilling stress during storage. A total of 110,110 unigenes were generated via high-throughput sequencing. Differentially expressed genes (DEGs) analysis showed that 18,681 genes were up-regulated and 21,983 genes were down-regulated in low temperature condition. Many DEGs were related to the cell membrane system, antioxidant enzymes, carbohydrate metabolism, and hormone metabolism, which are potentially associated with sweetpotato resistance to low temperature. The existence of DEGs suggests a molecular basis for the biochemical and physiological consequences of sweetpotato in low temperature storage conditions. Our analysis will provide a new target for enhancement of sweetpotato cold stress tolerance in postharvest storage through genetic manipulation.