Hedgehog signaling regulates regenerative patterning and growth inHarmonia axyridisleg
Hedgehog signaling regulates regenerative patterning and growth inHarmonia axyridisleg
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Hedgehog信号调节异色瓢虫腿的再生模式和生长
DOI：
10.1007/s00018-020-03631-7
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发表时间：
2020-09-09
影响因子：
8
通讯作者：
Shen，Jie
中科院分区：
文献类型：
--
作者：
Zhou，Hang;Ma，Zhongzheng;Shen，Jie
附肢再生在许多物种中得到了广泛的研究。与其他动物模型相比，异色异色藻具有生命周期短、易于饲养、再生能力强、含有系统的RNAi等优点，是研究附肢再生的模式生物。在这里，我们进行了转录组分析，然后进行了基因功能分析，以揭示h的分子机制。坐骨神经再生生长过程。DPP、Wg、DS/Ft/Hippo、Notch、EGFR和Hedgehog(HH)等信号通路在小腿再生和生长过程中均上调。其中，HH及其辅助受体Lrp2是再生腿正常构型和生长所必需的。典型的HH信号靶标对再生生长是必需的，但对再生腿的模式形成不是必需的。dpp、wg和腿发育相关基因包括rn、dac1和dll都受hhandlrp2调控，可能在腿的再生模式形成中起重要作用。
Appendage regeneration has been widely studied in many species. Compared to other animal models,Harmonia axyridishas the advantage of a short life cycle, is easily reared, has strong regeneration capacity and contains systemic RNAi, making it a model organism for research on appendage regeneration. Here, we performed transcriptome analysis, followed by gene functional assays to reveal the molecular mechanism ofH. axyridisleg regenerative growth process. Signaling pathways including Decapentaplegic (Dpp), Wingless (Wg), Ds/Ft/Hippo, Notch, Egfr, and Hedgehog (Hh) were all upregulated during the leg regenerative patterning and growth. Among these, Hh and its auxiliary receptor Lrp2 were required for the proper patterning and growth of the regenerative leg. The targets of canonical Hh signaling were required for the regenerative growth which contributes to the leg length, but were not essential for the pattern formation of the regenerative leg.dpp,wgand leg developmental-related genes includingrn,dacandDllwere all regulated byhhandlrp2and may play an essential role in the regenerative patterning of the leg.