Methanogenic Archaea and human periodontal disease
Methanogenic Archaea and human periodontal disease
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DOI：
10.1073/pnas.0308766101
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发表时间：
2004-04-20
影响因子：
11.1
通讯作者：
Relman, DA
中科院分区：
文献类型：
--
作者：
Lepp, PW;Brinig, MM;Relman, DA
古生菌已经从人类的结肠、阴道和口腔中分离出来，但尚未被确定为人类疾病的原因。在这项研究中，我们通过定量聚合酶链式反应揭示了牙周病的严重程度与龈下裂隙中古生菌小亚基核糖体RNA基因(SSU RDNA)的相对丰度之间的关系。此外，随着临床症状的改善，治疗部位古生菌小亚基rDNA的相对丰度降低。36%的牙周炎患者携带古生菌，仅限于牙周病患者的龈下部位。荧光原位杂交证实在这些部位存在古生菌细胞。病区的古菌群落主要由一个类似口腔甲烷短杆菌的系统型和一个与栖息在许多动物的肠道中的古生菌相关的独特的甲烷短杆菌亚群所主导。我们假设，在牙周炎期间，产甲烷菌参与了龈下缝隙中促进继发性发酵菌定植的合养关系。因为它们是潜在的替代同养伙伴，我们对没有古菌的更大密螺旋体种群地点的发现进一步支持了这一假说。
Archaea have been isolated from the human colon, vagina, and oral cavity, but have not been established as causes of human disease. In this study, we reveal a relationship between the severity of periodontal disease and the relative abundance of archaeal small subunit ribosomal RNA genes (SSU rDNA) in the subgingival crevice by using quantitative PCR. Furthermore, the relative abundance of archaeal small subunit rDNA decreased at treated sites in association with clinical improvement. Archaea were harbored by 36% of periodontitis patients and were restricted to subgingival sites with periodontal disease. The presence of archaeal cells at these sites was confirmed by fluorescent in situ hybridization. The archaeal community at diseased sites was dominated by a Methanobrevibacter oralis-like phylotype and a distinct Methanobrevibacter subpopulation related to archaea that inhabit the gut of numerous animals. We hypothesize that methanogens participate in syntrophic relationships in the subgingival crevice that promote colonization by secondary fermenters during periodontitis. Because they are potential alternative syntrophic partners, our finding of larger Treponema populations sites without archaea provides further support for this hypothesis.