Synaptic organisation of the pelvic ganglion in the guinea-pig
Synaptic organisation of the pelvic ganglion in the guinea-pig
复制标题
豚鼠骨盆神经节的突触组织
DOI：
10.1007/bf00213794
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发表时间：
2004
影响因子：
3.6
通讯作者：
Professor G. Burnstock
中科院分区：
文献类型：
--
作者：
R. Yokota;Professor G. Burnstock
用5-羟多巴胺在体标记肾上腺素能神经末梢，对豚鼠盆前神经节的神经元胞体、神经轮廓和突触进行了半定量电镜研究。神经节细胞分为三种主要类型：1）大多数（约70%）不含颗粒囊泡，可能是胆碱能成分; 2）含有大小均匀的大颗粒囊泡（80-110 nm），具有中等密度的颗粒和突出的晕圈; 3）含有大小可变的囊泡（60-150 nm），具有非常密集的偏心颗粒核心。一些非神经元的“含颗粒”的细胞，主要是小血管附近。约95%的神经节内的总轴突配置文件是胆碱能，其余5%是肾上腺素能。然而，99%的突触（即在神经细胞膜50 nm内具有突触前和突触后特化的轴突）是胆碱能的，1%是肾上腺素能的。只有三个例子的神经细胞体表现出胆碱能和肾上腺素能突触进行了观察。与椎旁和椎前神经节不同，盆神经节含有大量的轴-体突触。多达20%的有核神经元细胞的档案显示两个不同的核。
A semi-quantitative electron-microscopic study of neuronal cell bodies, nerve profiles and synapses in the anterior pelvic ganglia of the guinea-pig has been carried out following in vivo labelling of adrenergic nerve endings with 5-hydroxydopamine. Ganglion cells of three main types have been distinguished: 1) the majority (about 70%) not containing granular vesicles, probably cholinergic elements; 2) those containing large granular vesicles of uniform size (80–110 nm), with granules of medium density and prominent halos; and 3) those containing vesicles of variable size (60–150 nm), with very dense eccentrically placed granular cores. Some non-neuronal ‘granule-containing’ cells were present, mainly near small blood vessels. Some 95% of the total axon profiles within the ganglia were cholinergic, the remaining 5% were adrenergic. However, 99% of synapses (i.e. axons within 50 nm of nerve cell membrane with pre-and post-synaptic specialisations) were cholinergic, and 1 % were adrenergic. Only three examples of nerve cell bodies exhibiting both cholinergic and adrenergic synapses were observed. Unlike the para-and prevertebral ganglia, the pelvic ganglia contained large numbers of axo-somatic synapses. As many as 20% of the nucleated neuronal cell profiles displayed two distinct nuclei.