Cdyl: a new transcriptional co-repressor
Cdyl: a new transcriptional co-repressor
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DOI：
10.1038/sj.embor.embor917
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发表时间：
2003-09-01
期刊：
影响因子：
7.7
通讯作者：
Khochbin, S
中科院分区：
文献类型：
--
作者：
Caron, C;Pivot-Pajot, C;Khochbin, S
Cdyl（chromodomain-Y-like）是一种主要在小鼠精子发生过程中表达的含有chromodomain-Y-like的蛋白。在其羧基末端部分，存在与烯酰-CoA水合酶/异构酶的辅酶A（CoA）口袋同源的结构域，其在此显示能够结合CoA和组蛋白脱乙酰酶（HDAC）。它也有效地抑制转录。此外，Hdacl的结合抑制Cdyl结合CoA的能力，并且Cdyl-CoA相互作用仅在不存在HDAC的情况下发生。这些数据表明Cdyl主要是转录辅阻遏物。然而，细胞Hdac 1和Hdac 2的降解，如在这里观察到的，在延长精子细胞中，可能提供了一个无HDAC的环境，其中Cdyl可以结合CoA并参与这些细胞中发生的全局染色质重塑。
Cdyl (chromodomain-Y-like) is a chromodomain-containing protein that is predominantly expressed during mouse spermiogenesis. In its carboxy-terminal portion, there is a domain with homology to the coenzyme A (CoA) pocket of the enoyl-CoA hydratase/isomerase, which is shown here to be able to bind CoA and histone deacetylases (HDACs). It also efficiently represses transcription. Moreover, the binding of Hdac1 represses the ability of Cdyl to bind CoA, and a Cdyl - CoA interaction only occurs in the absence of HDACs. These data suggest that Cdyl is primarily a transcriptional co-repressor. However, the degradation of cellular Hdac1 and Hdac2, as observed here in the elongating spermatids, may provide an HDAC-free environment in which Cdyl could bind CoA and participate in the global chromatin remodelling that occurs in these cells.