Adhesion of human fibroblasts to root-end-filling materials
Adhesion of human fibroblasts to root-end-filling materials
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DOI：
10.1097/00004770-200309000-00014
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发表时间：
2003-09-01
影响因子：
4.2
通讯作者：
Lallier, T
中科院分区：
文献类型：
--
作者：
Camp, MA;Jeansonne, BG;Lallier, T
本研究。评价培养的人牙周韧带成纤维细胞和牙龈成纤维细胞与不同根端填充材料的附着性。虽然牙周韧带和牙龈成纤维细胞最初热切地附着在Geristore(R)上，但这些细胞对prooroot （TM）、Tytin(R)汞合金或SuperEBA（TM）没有明显的附着。在Geristore(R)上进一步培养后，牙周韧带和牙龈成纤维细胞的附着均得到改善，这些细胞增殖。相比之下，与prooroot （TM）、Tytin(R)汞合金或WSuperEBA（TM）孵育较长时间的细胞，没有观察到附着或增殖的改善。由于这两组成纤维细胞的附着特性是相同的，我们研究了细胞外基质受体家族（整合素）在牙龈成纤维细胞附着中的潜在作用。研究发现，牙龈成纤维细胞对I型胶原的附着依赖于alpha1beta1和alpha2beta1整合素，而它们对纤维连接蛋白和玻璃体连接蛋白rgd结合序列的附着被针对beta1和alphaV整合素亚基的抗体部分抑制。然而，牙龈成纤维细胞对Geristore(R)的附着并没有因为添加任何干扰附着的抗整合素抗体而减少。因此，牙龈成纤维细胞附着在Geristore(R)上，但这种附着是由整合素以外的机制介导的。
This study. evaluated the attachment of cultured explants of human periodontal ligament fibroblasts and gingival fibroblasts to different root-end-filling materials. Although periodontal ligament and gingival fibroblasts initially attached avidly to Geristore(R), these same cells displayed no significant attachment to ProRoot(TM), Tytin(R) amalgam, or SuperEBA(TM). With further incubation on Geristore(R), the attachment of both periodontal ligament and gingival fibroblasts improved and these cells proliferated. In contrast, no improvement in attachment or proliferation was observed for cells incubated for greater times with ProRoot(TM), Tytin(R) amalgam, or WSuperEBA(TM). Because the attachment characteristics of these two groups of fibroblasts were identical, we examined the potential role of the extracellular matrix family of receptors (integrins) on the attachment of gingival fibroblasts. Gingival fibroblast attachment to collagen type I was determined to be dependent on alpha1beta1 and alpha2beta1 integrins, whereas their attachment to the RGD-binding sequence of fibronectin and vitronectin was partially inhibited by antibodies to the beta1 and alphaV integrin subunits. However, attachment of gingival fibroblasts to Geristore(R) was not reduced by the addition of any of the attachment-perturbing anti-integrin antibodies examined. Thus, gingival fibroblasts attach to Geristore(R), but this attachment was mediated by mechanisms other than integrins.