Hollow Fe3O4 Nanoparticles Assisted Signal Amplification for High-Performance Redox Molecule Catalysis toward Sensitive Electrochemical Immunoassay
Hollow Fe3O4 Nanoparticles Assisted Signal Amplification for High-Performance Redox Molecule Catalysis toward Sensitive Electrochemical Immunoassay
复制标题
空心 Fe3O4 纳米颗粒辅助信号放大高性能氧化还原分子催化灵敏电化学免疫分析
DOI：
10.1149/2.0211707jes
复制
发表时间：
2017
影响因子：
3.9
通讯作者：
Chen Sanping
中科院分区：
文献类型：
--
作者：
Wang Zhen;Ren Zijun;Yu Hua;Han Jing;Xie Gang;Chen Sanping
在这项工作中，我们制备了中空的Fe3O4纳米粒子(Fe3O4HNPs)作为纳米电催化剂，以获得多功能的氧化还原信号标签，用于高效硫氨酸(THI)催化超灵敏蛋白质的检测。简单地说，制备了导电性好、比表面积大的Au-Thi-Au，以提高氧化还原分子(THI)的固定化量。同时，为了提高催化效率，Au-Thi-Au(氧化还原分子)进一步固定在Fe3O4HNPs(纳米电催化剂)表面。显然，所制备的Au-Thi-Au@Fe3O4HNPs作为信号标记结合了电化学活性、催化活性、磁响应性和纳米载体的优点，用于电化学免疫分析。重要的是，由于Fe3O4 HNPs的中空结构和高活性中心，Au-Thi-Au@Fe3O4 HNPs的信号标记即使在没有底物的情况下也表现出高性能的THI催化作用，从而为甲胎蛋白的电化学检测提供了一种高灵敏度的方法，最小可达0.033 pg mL−1。与传统的基于酶的信号放大相比，该免疫传感器避免了在测试液中添加任何底物，具有良好的稳定性、灵敏度和准确性。
In this work, we prepared hollow Fe 3 O 4 nanoparticles (Fe 3 O 4 HNPs) as nanoelectrocatalyst to obtain a multi-functionalized redox signal tag for high-performance thionine (Thi) catalysis toward ultrasensitive protein detection. Briefly, Au-Thi-Au with good conductivity and large surface area was prepared to enhance the immobilization amount of redox molecular (Thi). Simultaneously, to improve catalytic efficiency, Au-Thi-Au (redox molecule) was further anchored on the surface of Fe 3 O 4 HNPs (nanoelectrocatalyst). Obviously, the as-prepared Au-Thi-Au@ Fe 3 O 4 HNPs as signal tag combines the merits of electrochemical activity, catalytic activity, magnetic response as well as nanocarrier, which is employed in electrochemical immunoassay. Importantly, owing to the hollow structure and high activity site of Fe 3 O 4 HNPs, the signal tag of Au-Thi-Au@ Fe 3 O 4 HNPs exhibits high-performance Thi catalysis even in the absence of substrate, thus, giving a highly sensitive method for electrochemical detection of alpha fetoprotein (AFP) down to the 0.033 pg mL− 1. Compared with traditional enzyme-based signal amplification, the proposed immunosensor avoids the addition of any substrate in testing solution, and shows good stability, sensitivity and accuracy.