Identification of polygyne and monogyne fire ant colonies (Solenopsis invicta) by multiplex PCR of Gp-9 alleles
Identification of polygyne and monogyne fire ant colonies (Solenopsis invicta) by multiplex PCR of Gp-9 alleles
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DOI：
10.1007/s00040-003-0662-8
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发表时间：
2003-01-01
期刊：
影响因子：
1.3
通讯作者：
Porter, SD
中科院分区：
文献类型：
--
作者：
Valles, SM;Porter, SD
设计了一对寡核苷酸引物，用于区分火蚁单雌和多雌群体中的GP-9(B)和GP-9(B)等位基因。针对GP-9(B)等位基因的特异性引物扩增出517bp的扩增片段，针对GP-9(B)等位基因的特异扩增片段产生423bp的扩增片段。当两组引物复合时，纯合子单性蚂蚁产生单个517bp的扩增子(专用于GP-9(B))，而杂合杂合蚂蚁产生一个517bp的扩增子和一个423bp的扩增子(分别专用于gp-9(B)和gp-9(B))，这使得可以在一次反应中识别出gp-9等位基因。该方法在来自20个一夫多妻制群体和20个一夫多妻制群体的蚂蚁身上进行了测试，对这两种形式的区分准确率为100%。
Oligonucleotide primers were designed to discriminate between the Gp-9(B) and Gp-9(b) alleles found in monogyne and polygyne colonies of fire ant, Solenopsis invicta Buren. Primers specific for the Gp-9(B) allele produced a 517 bp amplicon and primers, specific for Gp-9(b) allele produced a 423 bp amplicon. When both sets of primers were multiplexed, homozygous monogyne ants produced a single 517 bp amplicon (specific for Gp-9(B)), whereas heterozygous polygyne ants produced one 517 bp amplicon and one 423 bp amplicon (specific for Gp-9(B) and Gp-9(b), respectively) which allowed the Gp-9 alleles to be discerned in a single reaction. This method was tested on ants from 20 monogyne colonies and 20 polygyne colonies and was 100% accurate in discriminating the two forms.