Jasmonate signalling drives time-of-day differences in susceptibility of Arabidopsis to the fungal pathogen Botrytis cinerea.
Jasmonate signalling drives time-of-day differences in susceptibility of Arabidopsis to the fungal pathogen Botrytis cinerea.
复制标题
DOI：
10.1111/tpj.13050
复制
发表时间：
2015-12
期刊：
影响因子：
--
通讯作者：
Denby KJ
中科院分区：
文献类型：
--
作者：
Ingle RA;Stoker C;Stone W;Adams N;Smith R;Grant M;Carré I;Roden LC;Denby KJ
生物钟是一种内部计时机制，使植物能够预测环境的定期变化，例如光明和黑暗，以及病原体和食草动物等生物挑战。在这里，我们证明植物生物钟影响对坏死性真菌病原体灰葡萄孢的易感性。根据一天中的接种时间，拟南芥植物对灰霉病菌表现出不同的敏感性。与夜间相比，黎明接种后的易感性降低在恒定光照条件下持续存在，并且在功能失调的时钟突变体中受到破坏，这证明了植物时钟在驱动一天中对灰霉病菌易感性的作用。在主观黎明时接种后，对灰霉病菌的易感性降低与防御反应的更快转录重编程有关，感染反应基因的门控明显。核心时钟调节因子的直接靶基因在转录因子中富集，这些转录因子在主观黎明时对感染的反应比主观夜晚更快，这表明时钟对防御信号网络的影响。此外，茉莉酸信号传导在拟南芥对灰霉病菌的节律易感性中起着至关重要的作用，在 jaz6 突变体中，在主观夜间丢失时对这种病原体的易感性增强。生物钟影响植物新陈代谢和对环境的反应，包括宿主对病原体的易感性。在这里，我们证明拟南芥在黎明时不太容易受到真菌接种的影响，因为防御转录网络被更快地激活。在黎明和夜间差异表达的基因对于核心时钟转录因子的已知直接目标高度富集，这表明时钟与防御调节网络之间的直接联系。
The circadian clock, an internal time‐keeping mechanism, allows plants to anticipate regular changes in the environment, such as light and dark, and biotic challenges such as pathogens and herbivores. Here, we demonstrate that the plant circadian clock influences susceptibility to the necrotrophic fungal pathogen, Botrytis cinerea. Arabidopsis plants show differential susceptibility to B. cinerea depending on the time of day of inoculation. Decreased susceptibility after inoculation at dawn compared with night persists under constant light conditions and is disrupted in dysfunctional clock mutants, demonstrating the role of the plant clock in driving time‐of‐day susceptibility to B. cinerea. The decreased susceptibility to B. cinerea following inoculation at subjective dawn was associated with faster transcriptional reprogramming of the defence response with gating of infection‐responsive genes apparent. Direct target genes of core clock regulators were enriched among the transcription factors that responded more rapidly to infection at subjective dawn than subjective night, suggesting an influence of the clock on the defence‐signalling network. In addition, jasmonate signalling plays a crucial role in the rhythmic susceptibility of Arabidopsis to B. cinerea with the enhanced susceptibility to this pathogen at subjective night lost in a jaz6 mutant. The circadian clock impacts plant metabolism and responses to the environment, including host susceptibility to pathogens. Here we demonstrate that Arabidopsis is less susceptible to fungal inoculation at dawn, because the defence transcriptional network is more rapidly activated. Genes differentially expressed at dawn and night were highly enriched for known direct targets of core clock transcription factors, suggesting direct links between the clock and the defence regulatory network.