Bradykinin-stimulated changes in inositol phosphate mass in renal papillary collecting tubule cells.
Bradykinin-stimulated changes in inositol phosphate mass in renal papillary collecting tubule cells.
复制标题
缓激肽刺激肾乳头集合管细胞中磷酸肌醇质量的变化。
DOI：
10.1016/0006-291x(87)91553-1
复制
发表时间：
1987
影响因子：
3.1
通讯作者：
Kirkwood,MT
中科院分区：
文献类型：
--
作者：
Shayman,JA;Kirkwood,MT
缓激肽对肾乳头状集合小管中多磷酸肌醇化学水平变化的影响进行了研究。通过酶促荧光测定法测定磷酸肌醇质量。缓激肽诱导增加肌醇单，双，三磷酸，这是时间和浓度依赖性。此外，形成的肌醇单磷酸的化学水平的大小不太可能仅由肌醇三磷酸的形成和降解来解释。这些观察结果与磷脂酰肌醇和磷脂酰肌醇二磷酸的伴随水解一致。本研究还证实，在新鲜分离的肾小管，观察到缓激肽诱导的磷脂酰肌醇二磷酸水解以前在放射性标记的文化。
The effect of bradykinin on changes in the chemical levels of myo-inositol polyphosphates in renal papillary collecting tubules was investigated. Myo-inositol phosphate mass was determined by means of an enzymatic, fluorometric assay. Bradykinin induced increases in myo-inositol mono-, bis-, and trisphosphate which were both time and concentration dependent. Furthermore, the magnitude of the chemical levels of myo-inositol monophosphate formed were unlikely to be accounted for solely by the formation and degradation of myo-inositol trisphosphate. These observations are consistent with the concomitant hydrolysis of phosphatidylinositol and phosphatidylinositol bisphosphate. This study also confirms, in freshly isolated renal tubules, observations regarding bradykinin-induced phosphatidyl-inositol bisphosphate hydrolysis made previously in radiolabeled cultures.