Genomics-based identification of targets in pathogenic bacteria for potential therapeutic and diagnostic use
Genomics-based identification of targets in pathogenic bacteria for potential therapeutic and diagnostic use
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DOI：
10.1016/s0300-483x(00)00454-6
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发表时间：
2001-03-07
期刊：
影响因子：
4.5
通讯作者：
Söll, D
中科院分区：
文献类型：
--
作者：
Raczniak, G;Ibba, M;Söll, D
许多完整的微生物基因组序列的可用性已经深刻地改变了我们对一些基本生物过程的理解。例如，参与氨基酸- trna （AA-tRNA）合成的酶，是负责蛋白质合成准确性的关键过程，已被发现具有高度的物种特异性。特别是，许多病原体含有某些与哺乳动物宿主无关的AA-tRNA合成途径。由于AA-tRNA的合成是细胞生存不可或缺的，因此发现病原体特异性途径和酶提供了新的治疗和诊断靶点。在这里，我们将回顾最近在阐明AA-tRNA合成途径方面的进展，并讨论这些发现可能的药物开发。特别是，基因组学和生物化学方法的整合，以确定治疗衣原体感染和莱姆病的诊断和治疗的新靶点。(C) 2001爱思唯尔科学爱尔兰有限公司版权所有。
The availability of numerous complete microbial genome sequences has profoundly altered our understanding of a number of fundamental biological processes. For example the enzymes involved in aminoacyl-tRNA (AA-tRNA) synthesis, the key process responsible for the accuracy of protein synthesis, have been found to be highly species-specific. In particular, a number of pathogens contain certain pathways of AA-tRNA synthesis that are unrelated to those found in their mammalian hosts. Since AA-tRNA synthesis is indispensable for cell viability, the discovery of pathogen-specific pathways and enzymes presents novel therapeutic and diagnostic targets. Here we will review recent advances in the elucidation of AA-tRNA synthesis pathways and discuss the possible pharmaceutical exploitation of these discoveries. In particular, the integration of genomic and biochemical approaches to identify novel targets for the treatment of Chlamydial infections and the diagnosis and treatment of Lyme disease will be presented. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.