Anatomical and physiological evidence for D1 and D2 dopamine receptor colocalization in neostriatal neurons
Anatomical and physiological evidence for D1 and D2 dopamine receptor colocalization in neostriatal neurons
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DOI：
10.1038/72929
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发表时间：
2000-03-01
影响因子：
25
通讯作者：
Aperia, A
中科院分区：
文献类型：
--
作者：
Aizman, O;Brismar, H;Aperia, A
尽管多巴胺信号很重要，但目前尚不清楚这两种主要的多巴胺受体亚类是否存在于相同或不同的神经元群体中。在这里，我们使用共聚焦显微镜来证明，几乎所有的纹状体神经元，无论是在体外还是在体内，都含有这两种类型的多巴胺受体。我们还提供了这种共定位的功能证据：在基本上所有被研究的神经元中，D-1受体亚类的激动剂非诺多巴抑制Na+/K+泵和河豚毒素(TTX)敏感的钠通道，而DR受体亚类的激动剂奎比罗激活TTX敏感的钠通道。因此，D-1和D-2类配体可能在功能上与基底节内几乎所有的多巴胺反应神经元相互作用。
Despite the importance of dopamine signaling, it remains unknown if the two major subclasses of dopamine receptors exist on the same or distinct populations of neurons. Here we used confocal microscopy to demonstrate that virtually all striatal neurons, both in vitro and in vivo, contained dopamine receptors of both classes. We also provide functional evidence for such colocalization: in essentially all neurons examined, fenoldopam, an agonist of the D-1 subclass of receptors, inhibited both the Na+/K+ pump and tetrodotoxin (TTX)-sensitive sodium channels, and quinpirole, an agonist of the Dr subclass of receptors, activated TTX-sensitive sodium channels. Thus D-1 and D-2 classes of ligands may functionally interact in virtually all dopamine-responsive neurons within the basal ganglia.