Structures of the stator complex that drives rotation of the bacterial flagellum.
Structures of the stator complex that drives rotation of the bacterial flagellum.
复制标题
驱动细菌鞭毛旋转的定子复合体的结构。
DOI：
10.1038/s41564-020-0788-8
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发表时间：
2020-12
影响因子：
28.3
通讯作者：
Lea SM
中科院分区：
文献类型：
--
作者：
Deme JC;Johnson S;Vickery O;Aron A;Monkhouse H;Griffiths T;James RH;Berks BC;Coulton JW;Stansfeld PJ;Lea SM
细菌鞭毛是原型典型的蛋白质纳米机器，包括一个旋转的螺旋桨连接到膜嵌入的电机复合体。马达由一个中心转子组成，转子周围环绕着定子单元，定子单元将穿过细胞质膜的离子流耦合到扭矩产生。在这里，我们提出了定子复合物的结构，从梭菌产孢，枯草芽孢杆菌和拟态弧菌，允许解释定子机制的广泛的数据体。结构揭示了一个意想不到的不对称A5 B2亚基组装，其中5个A亚基包围两个B亚基。与其他离子驱动马达的结构相比，表明这种A5B2结构对细菌系统是至关重要的，该细菌系统将离子流的能量耦合到远处产生机械功，并表明此类事件涉及马达结构中的旋转。
The bacterial flagellum is the proto-typical protein nanomachine and comprises a rotating helical propeller attached to a membrane-embedded motor complex. The motor consists of a central rotor surrounded by stator units that couple ion flow across the cytoplasmic membrane to torque generation. Here we present the structures of stator complexes from Clostridium sporogenes, Bacillus subtilis and Vibrio mimicus, allowing interpretation of the extensive body of data on stator mechanism. The structures reveal an unexpected asymmetric A5B2 subunit assembly in which the five A subunits enclose the two B subunits. Comparison to structures of other ion-driven motors indicates that this A5B2 architecture is fundamental to bacterial systems that couple energy from ion-flow to generate mechanical work at a distance, and suggests that such events involve rotation in the motor structures.