Use of Aeromonas spp. as General Indicators of Antimicrobial Susceptibility among Bacteria in Aquatic Environments in Thailand.
Use of Aeromonas spp. as General Indicators of Antimicrobial Susceptibility among Bacteria in Aquatic Environments in Thailand.
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DOI：
10.3389/fmicb.2016.00710
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发表时间：
2016
影响因子：
5.2
通讯作者：
Tamura Y
中科院分区：
文献类型：
--
作者：
Usui M;Tagaki C;Fukuda A;Okubo T;Boonla C;Suzuki S;Seki K;Takada H;Tamura Y
抗菌剂被广泛使用，不仅用于治疗人类感染，还用于治疗牲畜和养鱼场。东南亚国家的人类栖息地靠近水生环境。因此，这些地区的人口面临接触抗微生物药物耐药性细菌的风险，从而传播抗微生物药物耐药性基因（ARGs）。在这项研究中，我们分别于2013年和2014年在泰国的15个地点（5个Chao Phraya河地点，2个Chao Phraya河河口地点，3个Ta Chin河地点和5个城市运河地点）和12个地点（6个城市运河地点，2个养鸡场地点，2个养猪场地点，2个养猪场地点，随后在沼气厂处理）收集了水样。从水样中共分离到117株气单胞菌，这些细菌表现出不同的抗菌敏感性。值得注意的是，环境四环素浓度与分离的气单胞菌的四环素耐药率之间存在显著的相关性；然而，来自猪场的样本中四环素耐药性的浓度和比率都高于来自其他水生环境的样本。这些发现表明，在这些水生环境中观察到的高浓度抗菌素可能会选择ARGs。此外，它们表明气单胞菌属是监测水生环境中抗菌素耐药性的有效标志物。
Antimicrobials are widely used, not only for treating human infections, but also for treatment of livestock and in fish farms. Human habitats in Southeastern Asian countries are located in close proximity to aquatic environments. As such, the human populations within these regions are at risk of exposure to antimicrobial resistant bacteria, and thereby disseminating antimicrobial resistance genes (ARGs). In this study, we collected water samples from 15 sites (5 sites in Chao Phraya River, 2 sites at the mouth of Chao Phraya River, 3 sites in Ta Chin River, and 5 sites at city canals) and 12 sites (6 sites at city canals; 2 sites at chicken farms; 2 sites at pig farms; and 2 samples from sites at pig farms, which were subsequently treated at a biogas plant) in Thailand in 2013 and 2014, respectively. In total, 117 Aeromonas spp. were isolated from the water samples, and these organisms exhibited various antimicrobial susceptibility profiles. Notably, there was a significant correlation between the environmental concentration of tetracyclines and the rates of tetracycline resistance in the isolated Aeromonas spp.; however, both the concentration and rates of tetracycline resistance in samples derived from pig farms were higher than those of samples harvested from other aquatic environments. These findings suggest that the high concentrations of antimicrobials observed in these aquatic environments likely select for ARGs. Furthermore, they indicate that Aeromonas spp. comprise an effective marker for monitoring antimicrobial resistance in aquatic environments.