SEQUENCE COMPARISONS OF DEVELOPMENTALLY REGULATED COLLAGEN GENES OF CAENORHABDITIS-ELEGANS
SEQUENCE COMPARISONS OF DEVELOPMENTALLY REGULATED COLLAGEN GENES OF CAENORHABDITIS-ELEGANS
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DOI：
10.1016/0378-1119(89)90173-x
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发表时间：
1989-03-30
期刊：
影响因子：
3.5
通讯作者：
HIRSH, D
中科院分区：
文献类型：
--
作者：
COX, GN;FIELDS, C;HIRSH, D
对来自秀丽隐杆线虫的胶原基因col-6、col-7（部分）、col-8、col-14和col-19进行测序，并与先前测序的基因col-1和col-2进行比较。这些基因的长度在1.0和1.2 kb之间，并且每个基因包括一个或两个短内含子。假定的启动子区含有与真核TATA启动子元件相似的序列。两个不同的，保守的序列被发现在推定的启动子区域，分别是幼虫特异性基因Col-2和Col-6，以及主要的成人特异性基因Col-7和Col-19。胶原多肽的结构域结构相似：每个多肽含有两个三螺旋形成（Gly-X-Y）n结构域，一个为30-33个氨基酸（aa），另一个为127-132个aa。后一个结构域被1 - 3个短的（2-8 aa）非（Gly-X-Y）n片段中断，这些片段出现在每个多肽中相对保守的位置。所有多肽中的（Gly-X-Y）n结构域两侧都有一组半胱氨酸残基。根据氨基酸序列的相似性，这些基因可以分为三个家族。家族内的基因并不总是表现出相似的发育表达程序，这表明基因的结构和调控区域分别进化。基因中的密码子使用是高度不对称的，其中腺嘌呤出现在85%的甘氨酸密码子的第三位，而脯氨酸密码子的第三位为93%。
Collagen genes col-6, col-7 (partial), col-8, col-14 and col-19 from the nematode Caenorhabditis elegans were sequenced, and compared to the previously sequenced genes col-1 and col-2. The genes are between 1.0 and 1.2 kb in length, and each includes one or two short introns. The presumptive promoter regions contain sequences similar to the eukaryotic TATA promoter element. Two distinct, conserved sequences were found in the presumptive promoter regions of, respectively, the dauer larva-specific genes col-2 and col-6, and the primarily adult-specific genes col-7 and col-19. The domain structures of the collagen polypeptides are similar: each polypeptide contains two triple-helix forming (Gly-X-Y)n domains, one of 30-33 amino acids (aa), and the other of 127-132 aa. The latter domain is interrupted by one to three short (2-8 aa) non(Gly-X-Y)n segments that occur at relatively conserved locations in each polypeptide. Sets of cysteine residues flank the (Gly-X-Y)n domains in all of the polypeptides. The genes can be placed into three families based upon amino acid sequence similarities. Genes within a family do not always exhibit similar development expression programs, suggesting that structural and regulatory regions of the genes have evolved separately. The codon usage in the genes is highly asymmetrical, with adenine appearing in the third position of 85% of the glycine codons, and 93% of the proline codons.