Maternal factors required for oocyte developmental competence in mice Transcriptome analysis of non-surrounded nucleolus (NSN) and surrounded nucleolus (SN) oocytes
Maternal factors required for oocyte developmental competence in mice Transcriptome analysis of non-surrounded nucleolus (NSN) and surrounded nucleolus (SN) oocytes
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小鼠卵母细胞发育能力所需的母体因素 非包围核仁 (NSN) 和包围核仁 (SN) 卵母细胞的转录组分析
DOI：
10.4161/cc.24991
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发表时间：
2013-06-15
期刊：
影响因子：
4.3
通讯作者：
Sun, Qing-Yuan
中科院分区：
文献类型：
--
作者：
Ma, Jun-Yu;Li, Mo;Sun, Qing-Yuan
在小鼠窦卵泡发育过程中，卵母细胞染色质逐渐从核仁周围无Hoechst阳性边缘的低浓缩状态（NSN）转变为核仁周围有Hoechst阳性边缘的完全浓缩状态（SN）。与SN卵母细胞相比，NSN卵母细胞基因转录活性高，减数分裂恢复（G（2）/M转换）率低，体外受精后大多停滞在二细胞期。为了探讨NSN和SN卵母细胞之间的差异，以及卵母细胞发育能力所需的母体因素，我们比较了NSN和SN卵母细胞之间的全转录组谱。首先，我们发现NSN和SN卵母细胞的代谢途径不同。在磷脂酰肌醇信号通路中，SN卵母细胞倾向于产生二酰基甘油，而NSN卵母细胞倾向于产生磷脂酰肌醇（3，4，5）-三磷酸。在能量生产方面，SN卵母细胞和NSN卵母细胞在胚胎发育和合成过程中存在差异。其次，我们还发现与卵母细胞减数分裂和/或植入前胚胎发育相关的关键基因在NSN和SN卵母细胞中的表达存在差异。我们的研究结果表明，在NSN-SN过渡，卵母细胞改变其代谢活动，并积累母源因子，为卵母细胞的进一步成熟和受精后胚胎发育。
During mouse antral follicle development, the oocyte chromatin gradually transforms from a less condensed state with no Hoechst-positive rim surrounding the nucleolus (NSN) to a fully condensed chromatin state with a Hoechst-positive rim surrounding the nucleolus (SN). Compared with SN oocytes, NSN oocytes display a higher gene transcription activity and a lower rate of meiosis resumption (G(2)/M transition), and they are mostly arrested at the two-cell stage after in vitro fertilization. To explore the differences between NSN and SN oocytes, and the maternal factors required for oocyte developmental competence, we compared the whole-transcriptome profiles between NSN and SN oocytes. First, we found that the NSN and SN oocytes were different in their metabolic pathways. In the phosphatidylinositol signaling pathway, the SN oocytes tend to produce diacylglycerol, whereas the NSN oocytes tend to produce phosphatidylinositol (3,4,5)-trisphosphate. For energy production, the SN oocytes and NSN oocytes differed in the gluconeogenesis and in the synthesis processes. Second, we also found that the key genes associated with oocyte meiosis and/or preimplantation embryo development were differently expressed in the NSN and SN oocytes. Our results illustrate that during the NSN-SN transition, the oocytes change their metabolic activities and accumulate maternal factors for further oocyte maturation and post-fertilization embryo development.