Prolonged nitric oxide inhalation during recovery from chronic hypoxia does not decrease nitric oxide-dependent relaxation in pulmonary arteries
Prolonged nitric oxide inhalation during recovery from chronic hypoxia does not decrease nitric oxide-dependent relaxation in pulmonary arteries
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DOI：
10.1378/chest.126.6.1919
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发表时间：
2004-12-01
期刊：
影响因子：
9.6
通讯作者：
Miyasaka, K
中科院分区：
文献类型：
--
作者：
Maruyama, J;Jiang, BH;Miyasaka, K
研究目的：探讨长期吸入一氧化氮（NO）对慢性低氧性肺动脉高压大鼠右心室肥厚（RVH）恢复过程及肺动脉（PA）NO环磷酸鸟苷（cGMP）舒张通路功能改变的影响。将35只大鼠暴露于慢性低压缺氧（380 mm Hg，10%氧气），39只大鼠暴露于空气中10天。然后将两组暴露于NO 10 ppm、NO 40 ppm或空气中3天或10天（每种NO浓度的对照组），总共16组。在预收缩的PA环中评价乙酰胆碱和硝普钠（SNP）诱导的舒张。结果：在内皮完整的肺外动脉或肺内动脉高血压管道（IPA）中，吸入NO对RVH的消退及乙酰胆碱或SNP引起的舒张功能受损的恢复过程均无影响。在正常内皮完整的管道IPA中，吸入40 ppmNO 10天部分增强SNP诱导的舒张。结论：持续吸入NO不影响慢性缺氧后恢复期大鼠右肺动脉已建立的RVH和受损的内源性NO-cGMP舒张级联反应的消退过程。
Study objective: To investigate the effects of long-term nitric oxide (NO) inhalation on the recovery process of right ventricular hypertrophy (RVH) and functional alterations in the NO-cyciic guanosine monophosphate (cGMP) relaxation pathway in rat conduit pulmonary arteries (PAs) in established chronic hypoxic pulmonary hypertension.Materials and methods: A total of 35 rats were exposed to chronic hybaric hypoxia (380 mm Hg, 10% oxygen), and 39 rats were exposed to air for 10 days. Both groups were then exposed to 3 or 10 days of NO 10 ppm, NO 40 ppm, or air (control groups for each NO concentration), resulting in a total of 16 groups. Acetylcholine- and sodium nitroprusside (SNP)-induced relaxation were evaluated in precontracted PA rings. RVH was assessed by heart weight ratio of right ventricle to left ventricle plus septum.Results: NO inhalation had no effect on either the regression of RVH or the recovery process of iinpaired relaxation induced by acetylcholine or SNP in a endothelium-intact hypertensive conduit extrapulmonary artery or intrapulmonary artery (IPA). in a normal endothelium-intact conduit IPA, 40 ppm NO inhalation for 10 days partially augmented SNP-induced relaxation. but not that induced by acetylcholine.Conclusion: Continuous NO inhalation did not affect the regression process of either established RVH or the impaired endogenous NO-cGMP relaxation cascade in a conduit PA in rats during the recovery period after chronic hypoxia.