Association between the lack of teeth and the expression of myosins in masticatory muscles of microphthalmic mouse
Association between the lack of teeth and the expression of myosins in masticatory muscles of microphthalmic mouse
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小眼小鼠牙齿缺失与咀嚼肌肌球蛋白表达的关系
DOI：
10.1002/cbf.1821
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
A.
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作者：
NARIYAMA;M.;KOTA;Y.;KANEKO;S.;ASADA;Y. and YAMANE;A.
本研究旨在探讨缺牙对咬肌、颞肌、二腹肌等咀嚼肌的影响，并比较缺牙对咀嚼肌的影响。我们分析了由于自身基因位点突变而不能长牙的小眼（mi/mi）小鼠这些肌肉中肌球蛋白重链（MyHC）异构体信使RNA （mRNA）和蛋白质的表达。采用实时聚合酶链反应和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳技术分别检测+/+和mi/mimice的咬肌、颞肌、二腹肌、胫前肌和腓肠肌中MyHC mRNA和蛋白表达水平。与+/+组相比，8周龄大肌/大肌组MyHC新生儿mRNA和蛋白表达分别高出4.1倍（p< 0.05）和3.3倍（p< 0.01）；新生儿MyHC蛋白的表达量占大鼠咬肌MyHC蛋白总表达量的19%。在二腹肌中，MyHC - 1 mRNA和蛋白的表达量分别比+/+组高4.7倍（p< 0.05）和5倍（p< 0.01）。在颞肌、胫前肌和腓肠肌中，+/+和mi/mimice之间MyHC同种异构体mRNA和蛋白的表达水平无显著差异。这些结果表明牙齿缺失与咀嚼肌和二腹肌MyHC表达之间存在关联，但在颞肌中没有这种关联，这表明牙齿缺失的影响在咀嚼肌中是不同的。版权所有©2011 John Wiley & Sons， Ltd
The purposes of the present study were to elucidate the influences of the deficiency of teeth on masticatory muscles, such as the masseter, temporalis and digastric muscles and compare the influence among masticatory muscles. We analysed the expressions of myosin heavy chain (MyHC) isoform messenger RNA (mRNA) and protein in these muscles in the microphthalmic (mi/mi) mouse, whose teeth cannot erupt because of a mutation in themitfgene locus. The expression levels of MyHC mRNA and protein in the masseter, temporalis, digastric, tibialis anterior and gastrocnemius muscles of+/+andmi/mimice were analysed with real‐time polymerase chain reaction and sodium dodecyl sulfate‐polyacrylamide gel electrophoresis, respectively. Themi/mimasseter muscle at 8 weeks of age expressed 4·1‐fold (p< 0·05) and 3.3‐fold (p< 0·01) more MyHC neonatal mRNA and protein than that in the +/+, respectively; the expression level of MyHC neonatal protein was 19% of the total MyHC protein in the masseter muscle ofmi/mimice. In the digastric muscle, the expression levels of MyHC I mRNA and protein in themi/mimice were 4·7‐fold (p< 0·05) and 5‐fold (p< 0·01) higher than those in the+/+mice. In the temporalis, tibialis anterior and gastrocnemius muscles, there was no significant difference in the expression levels of any MyHC isoform mRNA and protein between+/+andmi/mimice. These results indicate associations between the lack of teeth and the expression of MyHC in the masseter and digastric muscles but not such associations in the temporalis muscle, suggesting that the influence of tooth deficiency varies among the masticatory muscles. Copyright © 2011 John Wiley & Sons, Ltd.