ASSEMBLY OF A PROCESSIVE MESSENGER-RNA POLYADENYLATION COMPLEX
ASSEMBLY OF A PROCESSIVE MESSENGER-RNA POLYADENYLATION COMPLEX
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DOI：
10.1002/j.1460-2075.1993.tb05690.x
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发表时间：
1993-02-01
期刊：
影响因子：
11.4
通讯作者：
WAHLE, E
中科院分区：
文献类型：
--
作者：
BIENROTH, S;KELLER, W;WAHLE, E
通过聚（A）聚合酶对mRNA前体的聚腺苷酸化取决于两个特异性因子及其识别序列。这些是裂解和聚腺苷酸化特异性因子（CPSF），识别聚腺苷酸信号AAUAAA和聚（A）结合蛋白II（PAB II），与生长的poly（a）尾巴相互作用。它们的效果与ATP无关，RNA 5'-CAP。通过非降解性凝胶电泳对RNA - 蛋白相互作用的分析表明，CPSF，PAB II和Poly（a）聚合酶与底物RNA形成季元复合物与底物RNA，可暂时稳定聚（a）聚合酶与RNA 3'-末端的结合。只有所有三种蛋白质形成的复合物才能有效地合成全长poly（a）尾巴。
Polyadenylation of mRNA precursors by poly(A) polymerase depends on two specificity factors and their recognition sequences. These are cleavage and polyadenylation specificity factor (CPSF), recognizing the polyadenylation signal AAUAAA, and poly(A) binding protein II (PAB II), interacting with the growing poly(A) tail. Their effects are independent of ATP and an RNA 5'-cap. Analysis of RNA-protein interactions by non-denaturing gel electrophoresis shows that CPSF, PAB II and poly(A) polymerase form a quaternary complex with the substrate RNA that transiently stabilizes the binding of poly(A) polymerase to the RNA 3'-end. Only the complex formed from all three proteins is competent for the processive synthesis of a full-length poly(A) tail.