LAP3 promotes glioma progression by regulating proliferation, migration and invasion of glioma cells
LAP3 promotes glioma progression by regulating proliferation, migration and invasion of glioma cells
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LAP3通过调节胶质瘤细胞的增殖、迁移和侵袭促进胶质瘤进展
DOI：
10.1016/j.ijbiomac.2014.10.021
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发表时间：
2015-01-01
影响因子：
8.2
通讯作者：
Wang, Dongling
中科院分区：
文献类型：
--
作者：
He, Xiaojuan;Huang, Qingfeng;Wang, Dongling
亮氨酸氨基肽酶3 （LAP3）属于M1家族，已被证明能催化亮氨酸残基的水解。亮氨酸氨基肽酶参与多种病理疾病，调节肿瘤细胞增殖、侵袭和/或血管生成。最近的研究表明，LAP3在多种恶性肿瘤中高表达，并影响肿瘤血管生成。我们旨在探讨LAP3在胶质瘤中的表达及其在胶质瘤细胞中的生物学功能。采用RT-PCR、Western blot、免疫组化等方法检测121例胶质瘤组织中LAP3的表达水平，发现LAP3高表达与胶质瘤患者恶性程度及预后不良相关。体外，在胶质瘤细胞中转染myc-LAP3增加LAP3，转染siRNA敲低LAP3后，采用CCK8实验、流式细胞术、创面愈合和transwell侵袭实验检测细胞活力、细胞周期、迁移和侵袭。结果表明，增加LAP3可促进细胞活力、细胞周期、迁移和侵袭，而敲低LAP3可降低细胞活力、抑制细胞增殖、迁移和侵袭。提示LAP3可能是一个新的预后因子，与胶质瘤细胞的生长、迁移、侵袭密切相关。(C) 2014 Elsevier B.V.版权所有
Leucine aminopeptidase 3 (LAP3), belonging to the M1 family, has been proved to catalyze the hydrolysis of leucine residues. Leucine aminopeptidases are involved in many pathological disorders and regulate cell proliferation, invasion and/or angiogenesis of tumor. Recent study showed that LAP3 is highly expressed in several malignant and affects tumor angiogenesis. We aimed at investigating the clinical significance of LAP3 expression in human gliomas and its biological function in glioma cells. RT-PCR, Western blot and immunohistochemistry analysis were used to detect the expression levels of LAP3 in 121 glioma tissues, high LAP3 expression was correlated with the grade of malignancy and poor prognosis of glioma patients. In vitro, after increasing of LAP3 by myc-LAP3 transfection and knockdown of LAP3 by siRNA transfection in glioma cells, cell viability, cell cycle, migration and invasion were determined with CCK8 assay, flow cytometry, wound healing and transwell invasion assays. The results indicated that increasing LAP3 could promte cell viability, cell cycle, migration and invasion, knockdown LAP3 could decrease cell viability, suppress cell proliferation, migration and invasion. Our findings uncover that LAP3 might be a new prognostic factor and be close correlation with glioma cell growth, magration, invasion. (C) 2014 Elsevier B.V. All rights reserved.