Chemosensory proteins from the proboscis of Mamestra brassicae
Chemosensory proteins from the proboscis of Mamestra brassicae
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DOI：
10.1093/chemse/25.5.541
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发表时间：
2000-10-01
期刊：
影响因子：
3.5
通讯作者：
Steinbrecht, RA
中科院分区：
文献类型：
--
作者：
Nagnan-Le Meillour, P;Cain, AH;Steinbrecht, RA
通过免疫印迹技术，利用针对天蚕蛾一般气味结合蛋白的抗体，在油菜蛾雄虫的鼻子提取液中免疫检测到了可溶性的低分子蛋白质。同样的抗体在鼻骨超薄切片上弱标记茎锥感受器的淋巴感受器和皮下间隙。描述了柱状锥形感受器的形态。免疫检测蛋白产生了几个N-末端序列，其中三个在结合实验中显示出与Brassicae信息素化合物的氚类似物很强的亲和力。克隆了编码这些序列的cDNAs，结果表明这些新蛋白与果蝇OS-D蛋白有关。它们被命名为化学感觉蛋白(CSP-MbraA1-CSP-MbraA5和CSP-MbraB1和CSP-MbraB2)，可能具有气味结合蛋白样功能。嗅觉和味觉器官的共同定位表明，生理作用取决于细胞环境。
Soluble, low molecular weight proteins were immunodetected in proboscis extracts of Mamestra brassicae males by Western blot, using antibodies raised against the general odorant-binding protein of the moth Antheraea polyphemus. The same antibodies weakly labelled the sensillum lymph and subcuticular space of sensilla styloconica on ultrathin sections of the proboscis. The morphology of sensilla styloconica is described. The immunodetected proteins yielded several N-terminal sequences, three of which showed strong affinity for tritiated analogues of pheromonal com pounds of M. brassicae in binding assays. The cDNAs coding for these sequences were cloned and it was shown that the new proteins are related to the OS-D protein of Drosophila. They are named chemosensory proteins (CSP-MbraA1-CSP-MbraA5 and CSP-MbraB1 and CSP-MbraB2) and may have an odorant-binding protein-like function. A common localization in both olfaction and taste organs suggests a physiological role depending on the cellular environment.