AML1, AML2, AND AML3, THE HUMAN MEMBERS OF THE RUNT DOMAIN GENE-FAMILY - CDNA STRUCTURE, EXPRESSION, AND CHROMOSOMAL LOCALIZATION
AML1, AML2, AND AML3, THE HUMAN MEMBERS OF THE RUNT DOMAIN GENE-FAMILY - CDNA STRUCTURE, EXPRESSION, AND CHROMOSOMAL LOCALIZATION
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DOI：
10.1006/geno.1994.1519
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发表时间：
1994-09-15
期刊：
影响因子：
4.4
通讯作者：
GRONER, Y
中科院分区：
文献类型：
--
作者：
LEVANON, D;NEGREANU, V;GRONER, Y
分离和鉴定了3个人类RANT结构域基因AML1、AML2和AML3。除了高度保守的RANT结构域的同源性外，在蛋白质的其他部分也观察到了广泛的序列相似性。这三个氨基酸都携带一个相同的推定的ATP结合位点-GRSGRGKS-，它们的C末端一半特别富含脯氨酸和丝氨酸残基。虽然已有人克隆了AML1cDNAs，但AML2是RANT结构域基因家族中一个未被描述的新成员，AML3被鉴定为小鼠PEB-P2αA的人类同源物。AML1的染色体定位被证实，而AML2和AML3分别被定位在染色体1p36和6p21。分析AML1和AML2在造血细胞系中的表达，发现了明显的表达模式。(C)1994年学术出版社。
cDNAs corresponding to three human rant domain containing genes, AML1, AML2, and AML3, were isolated and characterized. In addition to homology in the highly conserved rant domain, extensive sequence similarities were also observed in other parts of the proteins. All three carried an identical, putative ATP binding site -GRSGRGKS-, and their C-terminal halves were particularly rich in proline and serine residues. While AML1 cDNAs were cloned by others, AML2 represents a new member, not previously described, of the rant domain gene family, and AML3 was identified as the human homologue of mouse PEB-P2 alpha A. The chromosomal location of AML1 to chromosome 21q22 was confirmed, while AML2 and AML3 were mapped to chromosome regions 1p36 and 6p21, respectively. Analysis of AML1 and AML2 expression in hematopoietic cell lines revealed a distinct pattern of expression. (C) 1994 Academic Press, Inc.