Hematopoietic mutations in the zebrafish.
Hematopoietic mutations in the zebrafish.
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DOI：
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发表时间：
1996-12
期刊：
影响因子：
4.6
通讯作者：
B. Weinstein;A. Schier;S. Abdelilah;J. Malicki;L. Solnica-Krezel;D. Stemple;D. Stainier;F. Zwartkruis-F.-Z
中科院分区：
文献类型：
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作者：
B. Weinstein;A. Schier;S. Abdelilah;J. Malicki;L. Solnica-Krezel;D. Stemple;D. Stainier;F. Zwartkruis-F.-Z
我们已经确定了扰乱斑马鱼胚胎中第一个胚胎血细胞的形成或分化的突变。这些“原始”红细胞起源于躯干的中间细胞团，是背外侧板中胚层的衍生物。通过胚胎之间的输血，我们证明这组细胞为胚胎提供了所有或几乎所有的血细胞，直到受精后至少第 5 天。 ENU 诱变产生的幼虫致死突变影响这些细胞发育的不同步骤。有些会导致前体生成缺陷，有些会导致分化缺陷，有些会导致细胞光敏感性增加。
We have identified mutations that perturb the formation or differentiation of the first embryonic blood cells in the zebrafish embryo. These 'primitive' red blood cells originate in the intermediate cell mass of the trunk, a derivative of the dorsal lateral plate mesoderm. By transfusion of blood between embryos we demonstrate that this cohort of cells provides the embryo with all, or nearly all, of its blood cells until at least day 5 postfertilization. Larval lethal mutations generated by ENU mutagenesis affect different steps in the development of these cells. Some cause defects in precursor generation, others defects in differentiation, and others an increase in cellular photosensitivity.