Chromosomal "stress-response" domains govern the spatiotemporal expression of the bacterial virulence program.
Chromosomal "stress-response" domains govern the spatiotemporal expression of the bacterial virulence program.
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DOI：
10.1128/mbio.00353-15
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发表时间：
2015-04-28
期刊：
影响因子：
6.4
通讯作者：
Muskhelishvili G
中科院分区：
文献类型：
--
作者：
Jiang X;Sobetzko P;Nasser W;Reverchon S;Muskhelishvili G
最近的研究表明，维持正常细菌和致病性细菌的基因表达受染色体的结构动力学的控制，但是，机械装置的选择性表达具有适应性性状的选择性表达。 DNA的物理特性与自适应特征的表达之间的关系，包括病毒因子，在病原体dickeya dadantii（以前是erwinia chrysanthemi）中，在细菌感染期间遇到的不良条件下获得的转录组中，我们探索通过整合这些数据，在致病性控制中实现了丰富的DNA结构蛋白。连贯的基因表达在DNA热力学稳定性，超副丝动力学和病毒性状之间具有独特的耦合。
我们认为，具有特定功能的瞬态染色体结构域的组织是对环境压力的病原体的多功能性调整的基本手段。在理解细菌致病性的控制机制方面提供了突破。
Recent studies strongly suggest that the gene expression sustaining both normal and pathogenic bacterial growth is governed by the structural dynamics of the chromosome. However, the mechanistic device coordinating the chromosomal configuration with selective expression of the adaptive traits remains largely unknown. We used a holistic approach exploring the inherent relationships between the physicochemical properties of the DNA and the expression of adaptive traits, including virulence factors, in the pathogen Dickeya dadantii (formerly Erwinia chrysanthemi). In the transcriptomes obtained under adverse conditions encountered during bacterial infection, we explored the patterns of chromosomal DNA sequence organization, supercoil dynamics, and gene expression densities, together with the long-range regulatory impacts of the abundant DNA architectural proteins implicated in pathogenicity control. By integrating these data, we identified transient chromosomal domains of coherent gene expression featuring distinct couplings between DNA thermodynamic stability, supercoil dynamics, and virulence traits. We infer that the organization of transient chromosomal domains serving specific functions acts as a fundamental device for versatile adjustment of the pathogen to environmental stress. We believe that the identification of chromosomal “stress-response” domains harboring distinct virulence traits and mediating the cellular adaptive behavior provides a breakthrough in understanding the control mechanisms of bacterial pathogenicity.