Optical Control of Cell Signaling with Red/Far-Red Light-Responsive Optogenetic Tools in Caenorhabditis elegans
Optical Control of Cell Signaling with Red/Far-Red Light-Responsive Optogenetic Tools in Caenorhabditis elegans
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DOI：
10.1021/acssynbio.2c00461
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发表时间：
2023-02-20
影响因子：
4.7
通讯作者：
Aoki，Kazuhiro
中科院分区：
文献类型：
--
作者：
Oda，Shigekazu;Sato-Ebine，Emi;Aoki，Kazuhiro
光遗传学技术已广泛应用于线虫秀丽隐杆线虫以研究其神经功能。然而，由于大多数光遗传学对蓝光有响应，并且动物对蓝光表现出回避行为，因此人们热切期待对更长波长光响应的光遗传学工具的应用。在这项研究中，我们报告了 C 中的实现。 elegansof 是一种基于光敏色素的光遗传学工具，可响应红光/近红外光并操纵细胞信号传导。我们首先引入了SynPCB系统，该系统使我们能够合成光敏色素的生色团藻蓝蛋白（PCB），并证实了PCB在神经元、肌肉和肠道细胞中的生物合成。我们进一步证实，SynPCB 系统合成的 PCB 量足以进行光敏色素 B (PhyB)-光敏色素相互作用因子 3 (PIF3) 的光转换。此外，肠细胞内Ca2+水平的光遗传学升高诱导了排便运动程序。这些 SynPCB 系统和基于光敏色素的光遗传学技术对于阐明 C 的分子机制具有重要价值。 秀丽隐杆线虫的行为。
Optogenetic techniques have been intensively applied to the nematodeCaenorhabditis elegansto investigate its neural functions. However, as most of these optogenetics are responsive to blue light and the animal exhibits avoidance behavior to blue light, the application of optogenetic tools responsive to longer wavelength light has been eagerly anticipated. In this study, we report the implementation inC. elegansof a phytochrome-based optogenetic tool that responds to red/near-infrared light and manipulates cell signaling. We first introduced the SynPCB system, which enabled us to synthesize phycocyanobilin (PCB), a chromophore for phytochrome, and confirmed the biosynthesis of PCB in neurons, muscles, and intestinal cells. We further confirmed that the amount of PCBs synthesized by the SynPCB system was sufficient for photoswitching of phytochrome B (PhyB)-phytochrome interacting factor 3 (PIF3). In addition, optogenetic elevation of intracellular Ca2+levels in intestinal cells induced a defecation motor program. These SynPCB system and phytochrome-based optogenetic techniques would be of great value in elucidating the molecular mechanisms underlyingC. elegansbehaviors.