RECONSTRUCTION OF THE PHYLOGENY OF THE GENUS TRITICUM FROM VARIATION IN REPEATED NUCLEOTIDE-SEQUENCES
RECONSTRUCTION OF THE PHYLOGENY OF THE GENUS TRITICUM FROM VARIATION IN REPEATED NUCLEOTIDE-SEQUENCES
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DOI：
10.1007/bf00229502
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发表时间：
1992-07-01
影响因子：
5.4
通讯作者：
ZHANG, HB
中科院分区：
文献类型：
--
作者：
DVORAK, J;ZHANG, HB
通过对小麦属13个二倍体物种的系统发育研究，探讨了重复核苷酸序列变异作为系统发育研究工具的潜力。感觉上是鲍登。小麦属重复核苷酸序列家族的种内变异较低，表明Southern杂交中重复核苷酸序列的限制性片段图谱是各物种可靠和一致的特征。克隆的重复核苷酸序列与小麦属物种和外群长穗偃麦草（寄主）A的DNA的Southern印迹杂交。爱. Southern杂交放射自显影图中条带的存在或不存在被认为是系统发育分析的特征。一个最简约的树，解决了与PAUP版本3.0L计算机包。该树与该物种的细胞分类学和进化数据是一致的。
The potential of variation in repeated nucleotide sequences as a tool for phylogenetic studies was examined by investigating the phylogeny of 13 diploid species of the genus Triticum L. sensu Bowden. Low intraspecific variation in repeated nucleotide sequence families in Triticum indicated that restriction fragment profiles of repeated nucleotide sequences in Southern blots are reliable and uniform characteristics of each species. Cloned repeated nucleotide sequences were hybridized with Southern blots of DNAs of the Triticum species and the outgroup, Lophopyrum elongatum (Host) A. Love. The presence or absence of bands in the Southern blot autoradiograms was considered to be a character for phylogenetic analysis. A most parsimonious tree was resolved with the PAUP version 3.0L computer package. The tree was consistent with cytotaxonomic and evolutionary data available on the species.