Evaluation of enrichments of sulfate reducing bacteria from pristine hydrothermal vents sediments as potential inoculum for reducing trichloroethylene
Evaluation of enrichments of sulfate reducing bacteria from pristine hydrothermal vents sediments as potential inoculum for reducing trichloroethylene
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DOI：
10.1007/s11274-009-0136-x
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发表时间：
2010-01-01
影响因子：
4.1
通讯作者：
Garcia-Pena, E. I.
中科院分区：
文献类型：
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作者：
Guerrero-Barajas, C.;Garcia-Pena, E. I.
以乳酸和挥发性脂肪酸（VFAs）为底物，在硫酸盐还原条件下，对原始热液喷口沉积物富集物还原三氯乙烯（TCE）的能力进行了评价。TCE生物降解中间体顺式和反式1，2二氯乙烯对硫酸盐还原（SR）的影响也进行了评估。还研究了氰钴胺（CNB 12）和核黄素（RF）对三氯乙烯的SR和生物降解的影响。在37 ℃下培养来自喷口的沉积物，并补充4 g l（-1）SO 42-、乳酸盐或VFA，并在单独的实验中用CNB 12或RF进行相应的处理。以乳酸盐为底物，TCE去除率为86（150 μ mol l（-1）初始浓度），硫酸盐消耗率为48%。对于作为电子供体的VFA，TCE（300 μ mol l（-1）初始浓度）的去除率高达93%，硫酸根的去除率高达40%。乳酸盐和CNB 12的组合产生了最好的SR。总体结果表明，在这个微生物群落中，硫酸盐还原剂，脱卤，可能halorespiring细菌可能相互作用，并利用不同的底物发酵的互养协会，但没有中断的SR，尽管事实上，TCE总是存在的。同样清楚的是，在发生任何降解之前，必须在培养物中建立硫酸盐还原。这些热液喷口沉积物中存在的微生物群落可能是为脱氯目的设计的生物反应器的新接种物来源。
The evaluation of enrichments from pristine hydrothermal vents sediments on its capability of reducing trichloroethylene (TCE) under sulfate reducing conditions with lactate and volatile fatty acids (VFAs) as substrates was performed. Effect of the possible TCE biodegradation intermediates cis and trans 1,2 dichloroethenes on sulfate reduction (SR) was also evaluated. The influence of cyanocobalamin (CNB12) and riboflavin (RF) on the SR and biodegradation of TCE was also determined. Sediments from the vents were incubated at 37 degrees C and supplemented with 4 g l(-1) SO42-, lactate or VFAs and amended in the corresponding treatments with either CNB12 or RF in separated experiments. A percentage of TCE removal of 86 (150 mu mol l(-1) initial concentration) was attained coupled to 48% sulfate depletion with lactate as substrate. Up to 93% removal of TCE (300 mu mol l(-1) initial concentration) and 40% of sulfate was reached for VFAs as electron donor. A combination of lactate and CNB12 yielded the best SR. The overall results suggest a syntrophic association in this microbial community in which sulfate reducers, dehalogenating, and probably halorespiring bacteria may be interacting and taking advantage of the fermentation of substrates differently, but without interruption of SR in spite of the fact that TCE was always present. It was also clear that sulfate reduction must be established in the cultures before any degradation can occur. The microbial community present in these hydrothermal vents sediments could be a new source of inoculum for bioreactors designed for dechlorination purposes.