Genome-Wide Identification and Expression Analysis of the Biotin Carboxyl Carrier Subunits of Heteromeric Acetyl-CoA Carboxylase in Gossypium.
Genome-Wide Identification and Expression Analysis of the Biotin Carboxyl Carrier Subunits of Heteromeric Acetyl-CoA Carboxylase in Gossypium.
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棉异聚乙酰辅酶A羧化酶生物素羧基载体亚基的全基因组鉴定和表达分析
DOI：
10.3389/fpls.2017.00624
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发表时间：
2017
影响因子：
5.6
通讯作者：
Hua J
中科院分区：
文献类型：
--
作者：
Cui Y;Zhao Y;Wang Y;Liu Z;Ijaz B;Huang Y;Hua J
乙酰辅酶a羧化酶是一种重要的酶，它催化乙酰辅酶a的羧化反应生成丙二酰辅酶a，是质体中脂肪酸从头合成的重要步骤。本研究基于最近发表的棉花基因组数据，鉴定了24个棉花BCCP基因。其中，在raimondii、G. arboreum、G. hirsutum和G. barbadense中分别鉴定出4个、4个、8个和8个BCCP同源物。根据系统发育分析，这些基因被分为两类。在每一类中，这些同源物在基因结构和基序上相对保守。染色体分布表明，4种棉花的BCCP基因均均匀分布在相应的染色体或支架上。在毛棉和巴氏棉中，片段重复都是主要的重复事件。表达谱分析显示，8个GhBCCP基因在不同表达水平的组织中均有表达，其中II类GhBCCP基因主要在发育胚珠中表达。同时，对16个棉花BCCP基因的表达分析表明，这些基因分别受到冷胁迫和盐胁迫的诱导或抑制，且在不同组织中的表达模式存在差异。这些发现将有助于确定棉属植物BCCP基因的功能和进化特征。
Acetyl-CoA carboxylase is an important enzyme, which catalyzes acetyl-CoA’s carboxylation to produce malonyl-CoA and to serve as a committed step for de novo fatty acid biosynthesis in plastids. In this study, 24 putative cotton BCCP genes were identified based on the lately published genome data in Gossypium. Among them, 4, 4, 8, and 8 BCCP homologs were identified in Gossypium raimondii, G. arboreum, G. hirsutum, and G. barbadense, respectively. These genes were divided into two classes based on a phylogenetic analysis. In each class, these homologs were relatively conserved in gene structure and motifs. The chromosomal distribution pattern revealed that all the BCCP genes were distributed equally on corresponding chromosomes or scaffold in the four cotton species. Segmental duplication was a predominant duplication event in both of G. hirsutum and G. barbadense. The analysis of the expression profile showed that 8 GhBCCP genes expressed in all the tested tissues with changed expression levels, and GhBCCP genes belonging to class II were predominantly expressed in developing ovules. Meanwhile, the expression analysis for the 16 cotton BCCP genes from G. raimondii, G. arboreum and G. hirsutum showed that they were induced or suppressed by cold or salt stress, and their expression patterns varied among different tissues. These findings will help to determine the functional and evolutionary characteristics of the BCCP genes in Gossypium species.