Glutathione deficiency of the lower respiratory tract in patients with idiopathic pulmonary fibrosis
Glutathione deficiency of the lower respiratory tract in patients with idiopathic pulmonary fibrosis
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DOI：
10.1183/09031936.02.00262402
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发表时间：
2002-06-01
影响因子：
24.3
通讯作者：
Buhl, R
中科院分区：
文献类型：
--
作者：
Beeh, KM;Beier, J;Buhl, R
特发性肺纤维化（IPF）是一种病因不明的疾病。氧化负担和抗氧化剂的增加，如谷胱甘肽（GSH），下呼吸道缺乏，被认为在IPF的进展中起作用。痰诱导是研究呼吸道炎症的一种安全的无创工具。本研究的目的是评价诱导痰上清中谷胱甘肽的直接测量。16名IPF患者和15名健康、不吸烟的受试者进行了痰诱导。分光光度法测定痰液、唾液和血浆中总谷胱甘肽。与健康受试者相比，IPF患者的痰GSH降低了4倍以上（平均GSH 1.4 +/- 0.34 muM对5.8 +/- 0.98 muM）。所有受试者的唾液GSH普遍较低或检测不到。IPF患者血浆GSH水平较低（0.26 +/- 0.1对0.74 +/- 0.16）。在IPF患者中，痰GSH水平与病程和肺功能损害存在临界相关性。这些数据证实了氧化/抗氧化失衡在特发性肺纤维化发病机制中的既定作用。并显示诱导痰在特发性肺纤维化等间质性肺疾病中直接研究炎症过程和替代标志物的潜力。
Idiopathic pulmonary fibrosis (IPF) is a disease of unknown aetiology. Increased oxidant burden and antioxidant, e.g. glutathione (GSH), deficiency in the lower respirator), tract have been thought to play a role in the progression of IPF. Sputum induction is a safe noninvasive tool to study inflammation in the respiratory tract.The aim of the present study was to evaluate the direct measurement of GSH in induced sputum supernatant. Sixteen IPF patients and 15 healthy, nonsmoking subjects underwent sputum induction. Total GSH in sputum, saliva and plasma was measured spectrophotometrically.Sputum GSH was decreased more then four-fold in IPF patients when compared to healthy subjects (mean GSH 1.4 +/- 0.34 muM versus 5.8 +/- 0.98 muM). Salivary GSH was generally low or undetectable in all subjects. Plasma GSH levels were lower in IPF patients (0.26 +/- 0.1 versus 0.74 +/- 0.16 muM). In IPF patients, there was a borderline correlation of sputum GSH levels with disease duration and lung-function impairment.These data confirm the established role of oxidant/antioxidant imbalance in the pathogenesis of idiopathic pulmonary fibrosis. and show the potential of induced sputum to directly study inflammatory processes and surrogate markers in interstitial lung diseases like idiopathic pulmonary fibrosis.