Polyclonal activation of murine B lymphocytes by immune complexes.
Polyclonal activation of murine B lymphocytes by immune complexes.
复制标题
免疫复合物对小鼠 B 淋巴细胞的多克隆激活。
DOI：
10.4049/jimmunol.130.3.1066
复制
发表时间：
1983
影响因子：
4.4
通讯作者：
W. Weigle
中科院分区：
文献类型：
--
作者：
E. Morgan;W. Weigle
小鼠脾B淋巴细胞在同种免疫复合物刺激下增殖并分泌多克隆抗体。使用来自全血清的抗体或单克隆抗体形成复合物导致小鼠B淋巴细胞的刺激。抗体与抗原的比例似乎对多克隆抗体反应的产生至关重要。由于抗原和抗体在培养起始时独立添加，因此复合物的确切性质是未知的，但最佳的多克隆抗体形成发生在轻微的抗原过量时。免疫复合物诱导的多克隆抗体产生需要巨噬细胞和T细胞两者的存在，而B细胞增殖仅需要巨噬细胞。巨噬细胞的作用似乎是切割低m.w. (17 000）片段，其负责淋巴细胞活化。
Murine splenic B lymphocytes are stimulated by homologous immune complexes to proliferate and secrete polyclonal antibody. The use of antibody from whole serum or monoclonal antibodies to form complexes resulted in the stimulation of mouse B lymphocytes. The ratio of antibody to antigen appears to be critical for the generation of the polyclonal antibody response. Because antigen and antibody are added independently at culture initiation, the exact nature of the complex is unknown, but optimal polyclonal antibody formation occurs in slight antigen excess. Immune complex-induced polyclonal antibody production requires the presence of both macrophages and T cells, whereas B cell proliferation requires only macrophages. The role of the macrophage appears to be to cleave a low m.w. (17,000) fragment from the complex, which is responsible for lymphocyte activation.