Disruption of actin microfilaments causes cortical microtubule disorganization and extra-phragmoplast formation at M/G1 interface in synchronized tobacco cells.
Disruption of actin microfilaments causes cortical microtubule disorganization and extra-phragmoplast formation at M/G1 interface in synchronized tobacco cells.
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DOI：
10.1093/pcp/pch091
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发表时间：
2004-06
影响因子：
4.9
通讯作者：
A. Yoneda;Minori Akatsuka;F. Kumagai;S. Hasezawa
中科院分区：
文献类型：
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作者：
A. Yoneda;Minori Akatsuka;F. Kumagai;S. Hasezawa
在稳定表达gfp -微管蛋白融合蛋白的转基因烟草BY-2细胞中，研究了肌动蛋白微丝（MFs）在M/G1界面微管（MTs）组织中的作用。当MFs被BA处理破坏时，皮层MTs （CMT）在膜质体坍塌后3小时也没有重组，而未处理的细胞在1小时内完成了CMT重组。此外，在没有MFs的情况下，微管蛋白没有表现出适当的募集，而是留在了膜质体存在的位置，或者外膜质体被组织起来。这些膜外质体可以在功能上形成细胞外板。这是第一次观察到在一次核分裂过程中形成多个细胞板，以及与有丝分裂纺锤体或细胞核的位置无关的成片体的产生。讨论了这些观察结果对MFs在M/G1界面上的作用的意义。
The roles of actin microfilaments (MFs) in the organization of microtubules (MTs) at the M/G1 interface were investigated in transgenic tobacco BY-2 cells stably expressing a GFP-tubulin fusion protein, using the MF-disrupting agent, Bistheonellide A (BA). When MFs were disrupted by BA treatment, cortical MTs (CMTs) did not become reorganized even 3 h after phragmoplast collapse, whereas non-treated cells completed CMT reorganization within 1 h. Furthermore, in the absence of MFs, the tubulin proteins did not show appropriate recruitment but remained at the site where the phragmoplast had existed, or extra-phragmoplasts were organized. These extra-phragmoplasts could functionally form extra-cell plates. This is the first observation of the formation of multiple cell plates during one nuclear division, and of phragmoplast generation irrespective of the position of the mitotic spindle or nuclei. The significance of these observations on the role of MFs at the M/G1 interface is discussed.