Metabolically conditioned media derived from bone marrow stromal cells or human skin fibroblasts act as effective chemoattractants for mesenchymal stem cells.
Metabolically conditioned media derived from bone marrow stromal cells or human skin fibroblasts act as effective chemoattractants for mesenchymal stem cells.
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DOI：
10.1186/s13287-017-0664-5
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发表时间：
2017-09-29
影响因子：
7.5
通讯作者：
Rösen-Wolff A
中科院分区：
文献类型：
--
作者：
Gabrielyan A;Neumann E;Gelinsky M;Rösen-Wolff A
骨组织工程的主要目标是产生健康的骨骼以取代受损的组织。因此，需要优化的生物材料，使间充质干细胞能够存活和生长。到目前为止，基于细胞的组织工程骨植入物临床应用的主要挑战是氧气在组织中的扩散不良，这使得功能性血管网络成为必要。骨髓基质细胞（bone marrow stromal cells， BMSC）具有进化成不同细胞类型、在体外广泛扩增和释放旁分泌可溶性因子的能力，在组织工程中具有很高的吸引力。在过去的几年里，缺氧成为一种被证实的控制骨髓间充质干细胞增殖、分化和多能性的方法。在这里，我们应用不同的方法来表征代谢条件培养基（MCM）与缺氧条件培养基（HCM）的特性，并在二维迁移实验中评估它们吸引BMSC的能力。分离培养人源性骨髓间充质干细胞和成纤维细胞，获得HCM和MCM。两种培养基均采用血管生成阵列、细胞因子阵列和ELISA对选定因子进行表征。采用康宁Transwell®-96渗透性支撑室，采用孔径为8.0 μm的多孔聚酯膜进行二维迁移测试。HCM和MCM的表征显示MCM的血管生成因子浓度高于HCM。然而，MCM对BMSC的化学吸引能力与HCM相当。人皮肤成纤维细胞产生的HCM和MCM与人骨髓间充质干细胞产生的HCM和MCM同样能吸引人骨髓间充质干细胞。HCM和MCM对BMSC具有较高的化学吸引能力。这两种条件培养基都含有高浓度的血管生成因子，这些因子对血管生成和细胞迁移至关重要。这两种化学吸引条件介质也可以从皮肤成纤维细胞中获得，这在个体化治疗方法中很容易获得。
The main goal of bone tissue engineering has been the generation of healthy bone in order to replace affected tissue. Therefore, optimized biomaterials are needed which allow the survival and growth of mesenchymal stem cells. Until now the key challenge in the clinical application of cell-based tissue engineering bone implants was poor diffusion of oxygen into the tissue, making functional blood vessel networks a necessity. With their ability to evolve into different cell types, to expand extensively in vitro, and to release paracrine soluble factors, bone marrow stromal cells (BMSC) are highly attractive for tissue engineering. During the last years hypoxia became a proven method to control proliferation, differentiation, and pluripotency of BMSC. Here we applied different methods to characterize metabolically conditioned media (MCM) in comparison to hypoxia conditioned media (HCM) and evaluated their ability to attract BMSC in 2-D migration assays. BMSC and fibroblasts of human origin were isolated and cultivated to obtain HCM and MCM. Both media were characterized by angiogenesis arrays, cytokine arrays, and ELISA for selected factors. 2-D migration tests were performed with Corning Transwell®-96 permeable support chambers with porous polyester membranes with a pore size of 8.0 μm. Characterization of HCM and MCM revealed that the concentration of angiogenic factors was higher in MCM than in HCM. However, the chemoattractive capacity of MCM for BMSC was equivalent to that of HCM. HCM and MCM produced by human skin fibroblasts attracted human BMSC as efficiently as HCM and MCM produced by human BMSC. HCM and MCM have a high chemoattractive capacity for BMSC. Both conditioned media harbor high concentrations of angiogenic factors which are important for angiogenesis and cell migration. Both chemoattracting conditioned media can also be derived from skin fibroblasts which can easily be obtained from patients in individualized therapy approaches.
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影响因子：
3.7
作者：
Boomsma RA;Geenen DL
通讯作者：
Geenen DL
影响因子：
7.5
作者：
Vertelov G;Kharazi L;Muralidhar MG;Sanati G;Tankovich T;Kharazi A
通讯作者：
Kharazi A
影响因子：
3.3
作者：
Nie, Chunlei;Yang, Daping;Zhang, Jiewu
通讯作者：
Zhang, Jiewu
影响因子：
11.4
作者：
Nemajerova, Alice;Mena, Patricio;Petrenko, Oleksi
通讯作者：
Petrenko, Oleksi
DOI：
10.1111/j.1582-4934.2010.01157.x
发表时间：
2011-09-01
影响因子：
5.3
作者：
Spiropoulos, Antonia;Theodosaki, Maria;Goussetis, Evgenios
通讯作者：
Goussetis, Evgenios