Ultrasensitive determination of copper in complex biological media based on modulation of plasmonic properties of gold nanorods
Ultrasensitive determination of copper in complex biological media based on modulation of plasmonic properties of gold nanorods
复制标题
基于金纳米棒等离子体特性调节的复杂生物介质中铜的超灵敏测定
DOI：
10.1021/ac401789n
复制
发表时间：
2013
影响因子：
7.4
通讯作者：
Chen Yi
中科院分区：
文献类型：
--
作者：
Chen Shenna;Zhao Qian;Liu Fang;Huang Haowen;Wang Linqian;Yi Shoujun;Zeng Yunlong;Chen Yi
在复杂的生物介质，如细胞中的铜的准确测定是相当困难的，和铜调制的核-壳金纳米棒的形成的基础上的分析策略进行了描述。通过测量局部表面等离子体共振吸收的变化可以实现选择性和无标记感测。该技术可以测定微量的铜在人体血清，尿液和红细胞没有或与最小的样品预处理。人血清中铜的检出限为20.67 μM，人尿中铜的检出限为0.193 μM，单个细胞中铜的检出限为3.09 × 10 ~（-16）g。该技术具有选择性高、样品前处理简单或不需要样品前处理、无标记等优点。该方法的实际检测限可达2fM，仅需103个红细胞即可进行分析，该技术可扩展到单个细胞中痕量铜的检测。
Accurate determination of copper in complex biological media such as cells is quite difficult, and an analytical strategy based on copper-modulated formation of core–shell gold nanorods is described. Selective and label-free sensing can be achieved by measuring the change in the localized surface plasmon resonance absorption. The technique can determine trace amounts of copper in human serum, urine, and red blood cells without or with minimal sample pretreatment. The Cu detection limits are 20.67 μM in human serum, 0.193 μM in human urine, and 3.09 × 10–16g in a single cell. The advantages of the technique are the high selectivity, simple or no sample pretreatment, and label free. Boasting a practical detection limit down to 2 fM, only 103red blood cells are needed to conduct the analysis and the technique may be extended to the detection of trace amounts of copper in a single cell.