Hydration-driven transport of deformable lipid vesicles through fine pores and the skin barrier
Hydration-driven transport of deformable lipid vesicles through fine pores and the skin barrier
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DOI：
10.1016/s0006-3495(03)74917-0
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发表时间：
2003-02-01
影响因子：
3.4
通讯作者：
Gebauer, D
中科院分区：
文献类型：
--
作者：
Cevc, G;Gebauer, D
我们通过实验和理论研究了通过半透纳米多孔屏障的聚集体运输。通过测量和模拟跨越此类屏障的水合梯度的影响，证明囊泡形式的合适脂质聚集体的自发跨屏障运输是由应用部位的部分聚集体脱水驱动的。通过推广 Onsager 输运模型，我们推导出一组方程，使所有相关观察结果合理化。脱水引起的囊泡运动开始时有一个滞后时间。这对应于达到极限囊泡水合所需的时间；两者都与起始过量水量成正比，并随着应用现场相对湿度的增加而减少。跨屏障运输的速率对这些参数不敏感，但随着囊泡变形性和体积交换能力的增加而增加。这两种特性都取决于膜的成分。双层组分的可逆分层是非线性双层特性的原因，也可能影响膜的有效亲水性。囊泡表面的高亲水性和极端的聚集体形状适应性对于材料在皮肤上的成功运输是必要的。这证明了基础生物物理研究对于更好地理解生物系统以及在药物输送中实际使用人造的、受自然启发的载体的重要性。
We studied aggregate transport through semipermeable, nano-porous barriers experimentally and theoretically. By measuring and modeling the effect of hydration gradient across such barriers, spontaneous transbarrier transport of suitable lipid aggregates in vesicular form was proven to be driven by partial aggregate dehydration at the application site. By generalizing the Onsager transport model we derived a set of equations that rationalize all pertinent observations. Dehydration-induced vesicle motion starts with a lag time. This corresponds to the time needed to reach the limiting vesicle hydration; both are proportional to the starting excess water volume and decrease with increasing relative humidity at application site. The rate of transbarrier transport is insensitive to these parameters but increases with vesicle deformability and volume exchange capability. Both these properties depend on membrane composition. Reversible demixing of bilayer components is the cause of nonlinear bilayer characteristics and also potentially affects the effective membrane hydrophilicity. High hydrophilicity of vesicle surface and extreme aggregate shape adaptability together are necessary for successful material transport across the skin. This demonstrates the significance of basic biophysical investigations for better understanding of biological systems and for the practical use of artificial, nature-inspired carriers in drug delivery.